Growth of Listeria monocytogenes as a biofilm on various food-processing surfaces
Growth of Listeria monocytogenes as a biofilm on various food-processing surfaces
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DOI：
10.4315/0362-028x-59.8.827
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发表时间：
1996-08-01
影响因子：
2
通讯作者：
Frank, JF
中科院分区：
文献类型：
--
作者：
Blackman, IC;Frank, JF
本研究的目的是确定单核细胞增生李斯特菌作为生物膜在各种食品加工表面上生长的能力，包括不锈钢、Teflon（R）、尼龙和聚酯地板密封剂。当在胰蛋白胨大豆肉汤（TSB）中在21 ° C下孵育时，这些表面中的每一个都能够支持生物膜形成。聚酯地板密封剂上的生物膜形成最多（孵育7天后覆盖40%的表面积），尼龙上的生物膜形成最少（覆盖率为3%）。使用化学成分确定的基本培养基导致聚酯地板密封剂上缺乏生物膜形成，并且降低了不锈钢上的生物膜水平。在10摄氏度下孵育减少了生物膜的形成，但是在TSB中孵育18天后，Teflon和不锈钢仍然允许23至24%的覆盖。L.单核细胞增多症足以提供这种病原体污染食品加工厂环境的实质性风险，如果湿表面不保持在卫生条件下。
The objective of this research was to determine the ability of Listeria monocytogenes to grow as a biofilm on various food-processing surfaces including stainless steel, Teflon(R), nylon, and polyester floor sealant. Each of these surfaces was able to support biofilm formation when incubation was at 21 degrees C in Trypticase soy broth (TSB). Biofilm formation was greatest on polyester floor sealant (40% of surface area covered after 7 days of incubation) and least on nylon (3% coverage). The use of chemically defined minimal medium resulted in a lack of biofilm formation on polyester floor sealant, and reduced biofilm levels on stainless steel. Biofilm formation was reduced with incubation at 10 degrees C, but Teflon(R) and stainless steel still allowed 23 to 24% coverage after incubation in TSB for 18 days. Biofilm growth of L. monocytogenes was sufficient to provide a substantial risk of this pathogen contaminating the food-processing plant environment if wet surfaces are not maintained in a sanitary condition.