Expression and disruption of the Arabidopsis TOR (target of rapamycin) gene
Expression and disruption of the Arabidopsis TOR (target of rapamycin) gene
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DOI：
10.1073/pnas.092141899
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发表时间：
2002-04-30
影响因子：
11.1
通讯作者：
Robaglia, C
中科院分区：
文献类型：
--
作者：
Menand, B;Desnos, T;Robaglia, C
TOR（雷帕霉素靶标）蛋白激酶在酵母、哺乳动物和果蝇中被鉴定为响应营养和生长因子的细胞生长的中央控制器。在这里，我们表明，拟南芥拥有一个单一的 TOR 基因，编码一种能够与酵母 12-kDa FK506 结合蛋白和雷帕霉素复合的蛋白质，尽管拟南芥营养生长对雷帕霉素不敏感。对两个 T-DNA 插入突变体的分析表明，AtTOR 的破坏会导致胚乳和胚胎发育过早停滞。 T-DNA 介导的 AtTOR 与 GUS 报告基因的翻译融合使我们能够证明 AtTOR 在初级分生组织、胚胎和胚乳中表达，但在分化细胞中不表达。讨论了这些特征对植物 TOR 途径的影响。
TOR (target of rapamycin) protein kinases were identified in yeasts, mammals, and Drosophila as central controllers of cell growth in response to nutrient and growth factors. Here we show that Arabidopsis thaliana possesses a single TOR gene encoding a protein able to complex with yeast 12-kDa FK506-binding protein and rapamycin despite the insensitivity of Arabidopsis vegetative growth to rapamycin. Analysis of two T-DNA insertion mutants shows that disruption of AtTOR leads to the premature arrest of endosperm and embryo development. A T-DNA-mediated translational fusion of AtTOR with the GUS reporter gene allows us to show that AtTOR is expressed in primary meristem, embryo, and endosperm, but not in differentiated cells. The implications of these features for the plant TOR pathway are discussed.