Sex-specific differences in pain response by dopamine in the bed nucleus of the stria terminalis in rats
Sex-specific differences in pain response by dopamine in the bed nucleus of the stria terminalis in rats
复制标题
DOI：
10.1097/wnr.0b013e32835d8540
复制
发表时间：
2013-03-06
期刊：
影响因子：
1.7
通讯作者：
Kimura, Fukuko
中科院分区：
文献类型：
--
作者：
Hagiwara, Hiroko;Funabashi, Toshiya;Kimura, Fukuko
福尔马林伤害感受试验显示，在试验间期，疼痛反应存在特征性的性别差异。然而，这些差异背后的机制仍不清楚。我们最近报道了大鼠福尔马林实验中终纹床核外侧亚部（BSTL）的性别特异性参与。在这里，我们评估是否性别特异性差异的疼痛反应调制的多巴胺系统在BSTL。我们首先研究了注射多巴胺D1受体激动剂，dihydrexidine，或拮抗剂，SCH 23390，到BSTL的福尔马林试验的影响。在福尔马林试验的间歇期，注射D1受体激动剂对雄性或雌性大鼠没有影响。拮抗剂显着增强了雌性大鼠的伤害性反应，但在男性中没有，表明在福尔马林试验中多巴胺系统的参与性别差异。接下来，我们研究了多巴胺D1受体在BSTL中的表达。免疫组织化学分析表明，多巴胺D1受体表达的BSTL在两种性别，但表现出较强的免疫反应性，在雄性大鼠比在雌性。这些结果表明，在福尔马林试验中，多巴胺神经元投射到BSTL的反应在减轻雌性大鼠的疼痛中起作用，性别特异性差异。NeuroReport 24：181-185（C）2013年威科健康垂直酒吧Lippincott威廉姆斯&威尔金斯。神经报告2013，24：181-185
The formalin test for nociception shows characteristic sex differences in the pain response during the interphase period of the test. However, the mechanism underlying these differences remains unclear. We have recently reported the sex-specific involvement of the lateral subdivision of the bed nucleus of the stria terminalis (BSTL) in the formalin test in rats. Here, we evaluated whether sex-specific differences in the pain response were modulated by the dopamine system in the BSTL. We first examined the effects of injecting a dopamine D1 receptor agonist, dihydrexidine, or antagonist, SCH23390, into the BSTL on the formalin test. During the interphase of the formalin test, injection of the D1 receptor agonist exerted no effect in male or female rats. The antagonist significantly enhanced the nociceptive response in female rats but not in males, indicating a sex difference in the involvement of the dopamine system in the formalin test. Next, we examined the expression of dopamine D1 receptors in the BSTL. Immunohistochemical analysis showed that the dopamine D1 receptor was expressed in the BSTL in both sexes but showed stronger immunoreactivity in male rats than in females. These results suggest sex-specific differences in the formalin test in which the response of dopamine neurons projecting to the BSTL plays a role in attenuating pain in female rats. NeuroReport 24:181-185 (C) 2013 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. NeuroReport 2013, 24: 181-185