Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects
Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects
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DOI：
10.1111/j.1601-0825.2009.01522.x
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发表时间：
2009-05-01
期刊：
影响因子：
3.8
通讯作者：
Sasano, Y.
中科院分区：
文献类型：
--
作者：
Ebina, H.;Hatakeyama, J.;Sasano, Y.
本研究旨在建立一种临界大小的牙槽骨缺损的大鼠模型。12周龄大鼠右侧第一磨牙周围制作标准化的颊槽骨或近颊槽骨缺损模型，左侧作为对照。牙槽骨愈合情况采用三维计算机断层扫描进行定量检测。应用I型胶原原位杂交法检测牙槽骨缺损修复过程中成骨细胞和骨细胞的骨基质生成情况，24周时牙槽骨缺损明显修复，体积缩小88.3%，而近颊骨缺损修复较少。成骨细胞和骨细胞在3周时均有I型胶原的表达，但在6周后几乎不表达，使近颊缺损基本未修复。近颊缺损是一种临界大小的缺损，不能最终用骨修复。这可能是研究骨再生剂对人牙槽骨丢失效果的一种合适的实验模型。
This study was designed to establish a rat model of a critical size alveolar bone defect.Standardized buccal or mesiobuccal alveolar bone defects were made around the right first mandibular molar of 12-week-old rats, and the left was used as a control. Alveolar bone healing was examined quantitatively by three-dimensional micro-computed tomographic imaging. Bone matrix production of osteoblasts and osteocytes during repair of alveolar bone defects was examined with in situ hybridization for type I collagen.Buccal defects were repaired significantly and the volume decreased by 88.3% in week 24, whereas mesiobuccal defects were repaired little. Osteoblasts and osteocytes expressed type I collagen in both defects in week 3 but showed little expression by week 6 and thereafter, leaving the mesiobuccal defects largely unrepaired.The mesiobuccal defect is a critical-size defect that is not ultimately repaired with bone. It may be an appropriate experimental model for investigating the effectiveness of bone regenerative agents in human alveolar bone loss.