Salicylic and jasmonic acid pathways are necessary for defence against Dickeya solani as revealed by a novel method for Blackleg disease screening of invitro grown potato
Salicylic and jasmonic acid pathways are necessary for defence against Dickeya solani as revealed by a novel method for Blackleg disease screening of invitro grown potato
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DOI：
10.1111/plb.12339
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发表时间：
2015-09-01
期刊：
影响因子：
3.9
通讯作者：
Andreasson, E.
中科院分区：
文献类型：
--
作者：
Burra, D. D.;Muhlenbock, P.;Andreasson, E.
马铃薯是保障欧洲粮食安全的主要作物，黑胫病日益造成产量和储存期间的损失。最近，一种黑胫病病原体，即立枯狄克氏菌已被证明在北方欧洲蔓延，导致侵袭性疾病的发展。目前，鉴定耐受性商业马铃薯品种一直不成功，这是混淆了复杂的病因学的疾病和强大的环境影响疾病的发展。目前缺乏有效的测试系统。在这里，我们描述了一个系统，用于量化黑胫病症状的无菌试管马铃薯植株，这节省了时间和空间相比，温室和现有的田间试验。我们没有发现所描述的基于体外的筛选方法和现有的温室试验之间感染差异的证据。该系统有助于有效筛选马铃薯植株的黑胫病反应，而不依赖于其他微生物和可变的环境条件。因此，我们使用体外筛选方法，通过分析激素相关（水杨酸和茉莉酸）转基因马铃薯植物的疾病反应来增加对黑胫病发展中涉及的植物机制的理解。我们表明，茉莉酸（JA）和水杨酸（SA）途径调节马铃薯黑胫病的耐受性，这一结果与以前的研究结果不同，拟南芥防御反应necrotrophic细菌。我们证实了这一点，通过显示诱导SA标记，病程相关蛋白1（StPR1），和JA标记，脂氧合酶（StLOX），在Dickeya solani感染的离体马铃薯植株。我们还观察到，转基因马铃薯植株的块茎更容易发生软腐病相比，野生型，这表明SA和JA途径的作用，一般耐受Dickeya。
Potato is major crop ensuring food security in Europe, and blackleg disease is increasingly causing losses in yield and during storage. Recently, one blackleg pathogen, Dickeya solani has been shown to be spreading in Northern Europe that causes aggressive disease development. Currently, identification of tolerant commercial potato varieties has been unsuccessful; this is confounded by the complicated etiology of the disease and a strong environmental influence on disease development. There is currently a lack of efficient testing systems. Here, we describe a system for quantification of blackleg symptoms on shoots of sterile invitro potato plants, which saves time and space compared to greenhouse and existing field assays. We found no evidence for differences in infection between the described invitro-based screening method and existing greenhouse assays. This system facilitates efficient screening of blackleg disease response of potato plants independent of other microorganisms and variable environmental conditions. We therefore used the invitro screening method to increase understanding of plant mechanisms involved in blackleg disease development by analysing disease response of hormone- related (salicylic and jasmonic acid) transgenic potato plants. We show that both jasmonic (JA) and salicylic (SA) acid pathways regulate tolerance to blackleg disease in potato, a result unlike previous findings in Arabidopsis defence response to necrotrophic bacteria. We confirm this by showing induction of a SA marker, pathogenesis-related protein 1 (StPR1), and a JA marker, lipoxygenase (StLOX), in Dickeya solani infected invitro potato plants. We also observed that tubers of transgenic potato plants were more susceptible to soft rot compared to wild type, suggesting a role for SA and JA pathways in general tolerance to Dickeya.