PLASMA CORTISONE IN MAN - ITS DETERMINATION, PHYSIOLOGICAL VARIATION, AND SIGNIFICANCE
PLASMA CORTISONE IN MAN - ITS DETERMINATION, PHYSIOLOGICAL VARIATION, AND SIGNIFICANCE
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DOI：
10.1177/000456328001700502
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
CASHMORE, GC
中科院分区：
文献类型：
--
作者：
FEW, JD;CASHMORE, GC
在同时测定可的松、皮质醇和皮质酮的方法中，使用纸层析分离提取的类固醇，并通过竞争性蛋白结合进行测定。该方法的可靠性的证据，特别是相对于可的松的测定，提出。只有轻微的变化，皮质醇水平检测到响应运动，手术，和促肾上腺皮质激素，即使皮质醇水平的显着变化，同时记录。血浆可的松水平的惯性可能与其与血浆蛋白的结合程度较低有关，相对于皮质醇，这将允许更快的肝脏摄取和可能的大量血管外可的松池的蓄积。
In a method for the simultaneous determination of cortisone, cortisol and corticosterone, the extracted steroids are separated using paper chromatography and assayed by competitive protein binding. Evidence of the reliability of the method, particularly with respect to the determination of cortisone, is presented. Only trivial changes in cortisone level were detected in response to exercise, surgery, and ACTH, even though marked changes in cortisol level were simultaneously recorded. The inertia in plasma cortisone level may be related to its lesser degree of binding to plasma proteins, relative to cortisol, which would permit more rapid hepatic uptake and possible accumulation of a large extravascular pool of cortisone.