Interactions of intermediate filament protein synemin with dystrophin and utrophin
Interactions of intermediate filament protein synemin with dystrophin and utrophin
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DOI：
10.1016/j.bbrc.2006.05.192
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发表时间：
2006-08-04
影响因子：
3.1
通讯作者：
Robson, Richard M.
中科院分区：
文献类型：
--
作者：
Bhosle, Rahul C.;Michele, Daniel E.;Robson, Richard M.
联蛋白是一种独特的、非常大的中间纤维（IF）蛋白，存在于所有类型的肌肉细胞中，它与主要的中间纤维蛋白desmin和/或vimentin形成异聚中间纤维（IF）。我们在此表明，组织纯化的禽联蛋白直接与肌营养不良蛋白和肌营养不良蛋白相互作用，并且哺乳动物（人类）联蛋白同种异构体（α -联蛋白和β -联蛋白）的特定表达区域直接与肌营养不良蛋白和肌营养不良蛋白分子的特定表达域/区域相互作用。哺乳动物赛门明也被证明与肌营养不良蛋白在肌肉细胞培养物中共定位。这些结果表明，synemin是肌肉细胞中一种重要的IF蛋白，有助于加强细胞肌原纤维外周层与沿肌膜的沿海区域以及位于神经肌肉和肌腱连接处（NMJs和MTJs）的肌膜区域之间的联系。(c) 2006爱思唯尔公司版权所有。
Synemin is a unique, very large intermediate filament (IF) protein present in all types of muscle cells, which forms heteropolymeric intermediate filaments (IFs) with the major IF proteins desmin and/or vimentin. We show herein that tissue-purified avian synemin directly interacts with both dystrophin and utrophin, and that specific expressed regions of both of the mammalian (human) synemin isoforms (alpha-synemin and beta-synemin) directly interact with specific expressed domains/regions of the dystrophin and utrophin molecules. Mammalian synemin is also shown to colocalize with dystrophin within muscle cell cultures. These results indicate that synemin is an important IF protein in muscle cells that helps fortify the linkage between the peripheral layer of cellular myofibrils and the costameric regions located along the sarcolemma and the sarcolemma region located within the neuromuscular and myotendinous junctions (NMJs and MTJs). (c) 2006 Elsevier Inc. All rights reserved.