Argonaute-mediated translational repression (and activation)
Argonaute-mediated translational repression (and activation)
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DOI：
10.4161/fly.3.3.9025
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发表时间：
2009-07
期刊：
影响因子：
1.2
通讯作者：
Shintaro Iwasaki;Yukihide Tomari
中科院分区：
文献类型：
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作者：
Shintaro Iwasaki;Yukihide Tomari
microRNA（miRNAs）通过诱导翻译抑制和/或mRNA降解下调其靶基因的表达。在特定条件下，miRNAs甚至可以激活其靶mRNA的翻译。这些过程通过miRNA-蛋白质复合物或RNA诱导的沉默复合物（RISC）发生，其含有Argonaute（Ago）亚家族蛋白作为核心组分。然而，miRNA介导的翻译调控的详细机制仍不清楚。我们最近报道，在果蝇中，两种Ago蛋白，Ago 1和Ago 2，都可以抑制靶mRNA的翻译，但通过显着不同的机制。此外，我们在这里表明，Ago 2，而不是Ago 1，可以激活翻译的目标mRNA时，他们缺乏聚（A）尾，这表明聚（A）尾的长度是一个重要的决定因素的Ago 2功能的后果。本文就miRNAs如何根据这些新发现调节翻译作一综述。
microRNAs (miRNAs) down-regulate the expression of their target genes by inducing translational repression and/or mRNA decay. Under specific conditions, miRNAs can even activate translation of their target mRNAs. These processes occur via miRNA-protein complexes, or RNA-induced silencing complexes (RISCs), which contain Argonaute (Ago) subfamily protein as a core component. However, detailed mechanisms of miRNA-mediated translational regulation remain unclear. We recently reported that, in Drosophila, both of the two Ago proteins, Ago1 and Ago2, can repress translation of the target mRNAs, but by remarkably different mechanisms. Furthermore, we here show that Ago2, but not Ago1, can activate translation of the target mRNAs when they lack the poly(A) tail, suggesting that the length of poly(A) tail is an important determinant for the consequences of Ago2 function. This review focuses on how miRNAs regulate translation in light of these new findings.