Sympathetic and hyperthermic reactions by orexin A: Role of cerebral catecholaminergic neurons
Sympathetic and hyperthermic reactions by orexin A: Role of cerebral catecholaminergic neurons
复制标题
DOI：
10.1016/j.regpep.2006.10.002
复制
发表时间：
2007-03-01
影响因子：
--
通讯作者：
De Luca, V.
中科院分区：
文献类型：
--
作者：
Monda, M.;Viggiano, An.;De Luca, V.
本实验测试了脑内注射食欲素a引起的脑儿茶酚胺能神经元损伤对交感神经和产热效应的影响。交感神经对肩关节间棕色脂肪组织（IBAT）和MAT的放电率。在向侧脑室注射食欲素A （1.5 nmol）前和注射后150 min，监测聚氨酯麻醉雄性sd大鼠的结肠温度和心率。实验前3天，给大鼠静脉注射6-羟多巴胺（6-OHDA）或6-OHDA加地西帕明或生理盐水。结果表明，食欲素A能提高生理盐水预处理大鼠的交感神经放电率、IBAT、结肠温度和心率。这种增加可通过单独使用6-OHDA或6-OHDA加地西帕明进行预处理而被阻断。这些发现表明，大脑儿茶酚胺能神经元（特别是多巴胺能通路）在与食欲能系统激活相关的复杂反应中起着重要作用。(c) 2006 Elsevier B.V.版权所有
This experiment tested the effect of a lesion of cerebral catecholaminergic neurons on the sympathetic and thermogenic effects induced by an intracerebroventicular (icv) injection of orexin A. The firing rates of the sympathetic nerves to the interscapular brown adipose tissue (IBAT), along with MAT, colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle and over a period of 150 min after the injection. Three days before the experiment, the rats were pre-treated with an icv injection of 6-hydroxydopamine (6-OHDA) or 6-OHDA plus desipramine or saline. The results show that orexin A increases the sympathetic firing rate, IBAT, colonic temperatures and heart rate in the rats pre-treated with saline. This increase is blocked by the pre-treatment with 6-OHDA alone or 6-OHDA plus desipramine. These findings indicate that cerebral catecholaminergic neurons (particularly the dopaminergic pathway) play a fundamental role in the complex reactions related to activation of the orexinergic system. (c) 2006 Elsevier B.V. All rights reserved.