Relationship between maternal and child cytokine responses to allergen and phytohaemagglutinin 2 years after delivery
Relationship between maternal and child cytokine responses to allergen and phytohaemagglutinin 2 years after delivery
复制标题
DOI：
10.1111/j.1365-2249.2006.03083.x
复制
发表时间：
2006-06-01
影响因子：
4.6
通讯作者：
Troye-Blomberg, M.
中科院分区：
文献类型：
--
作者：
Larsson, A. -K.;Nilsson, C.;Troye-Blomberg, M.
目前对父母和孩子细胞因子谱之间的关系知之甚少。在这项研究中，我们旨在调查 2 岁儿童及其相应母亲在分娩 2 年后的细胞因子谱。从 IgE 致敏 (n = 15) 和非致敏 (n = 58) 2 岁儿童及其母亲中分离外周血单核细胞 (PBMC)。使用酶联免疫斑点 (ELISpot) 技术研究了对卵清蛋白、猫和植物血凝素 (PHA) 的反应。计数产生干扰素(IFN)-γ-、白细胞介素(IL)-4-、IL-10-和IL-12的细胞。 2 岁时，IgE 致敏的儿童对 PHA 的反应表现出产生 IL-4 的细胞数量增加，并且对过敏原产生 IL-10 和 IL-12 的细胞也有所增加，这对卵清蛋白的反应比对猫的反应更明显。在 IgE 致敏儿童的母亲中，无论其自身的特应性状态如何，发现对过敏原产生 IFN-γ、IL-10 和 IL-12 的细胞数量显着增加，但对 PHA 没有增加。母亲和孩子之间的 IgE 水平和细胞因子反应相关，表明细胞因子对过敏原和 PHA 的反应可能受到遗传因素的控制。我们推测，与母亲的过敏状态相反，细胞因子对过敏原的反应增加可能是儿童在生命早期发生 IgE 致敏的更好的预测因子和/或风险因素。
Little is known at present about the relation between parental and child cytokine profiles. In this study we aimed to investigate the cytokine profile of 2-year-old children and their corresponding mothers, 2 years after delivery. Peripheral blood mononuclear cells (PBMC) were isolated from IgE-sensitized (n = 15) and non-sensitized (n = 58) 2-year-old children and their mothers. The responses to ovalbumin, cat and phytohaemagglutinin (PHA) were investigated using the enzyme-linked immunospot (ELISpot) technique. Interferon (IFN)-gamma-, interleukin (IL)-4-, IL-10- and IL-12-producing cells were enumerated. At 2 years of age, IgE-sensitized children exhibited increased numbers of IL-4-producing cells in response to PHA and also showed an increase in IL-10- and IL-12-producing cells to allergen that was more pronounced in response to ovalbumin than to cat. A statistically significant increase in the numbers of IFN-gamma-, IL-10- and IL-12-producing cells to the allergens, but not to PHA, was found in the mothers of IgE-sensitized children irrespective of their own atopic status. IgE levels and cytokine responses were correlated between the mothers and their children, indicating that cytokine responses to both allergen and PHA might be governed by genetic factors. We speculate that the increased cytokine response to allergen, as opposed to the allergic status of the mother, might be a better predictor and/or a risk factor for the child to develop IgE-sensitization in early life.