B cell helper factors. I. Requirement for both interleukin 2 and another 40,000 mol wt factor.
B cell helper factors. I. Requirement for both interleukin 2 and another 40,000 mol wt factor.
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DOI：
10.1084/jem.154.5.1681
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发表时间：
1981-11-01
期刊：
影响因子：
--
通讯作者：
Kappler JW
中科院分区：
文献类型：
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作者：
Leibson HJ;Marrack P;Kappler JW
一种不同于白细胞介素2 （IL-2）的辅助因子(s)被证明存在于正常小鼠脾细胞（正常Con A Sn）的豆豆蛋白A刺激的上清中。完全耗尽T细胞的脾脏细胞需要IL-2和该因子来产生抗体分泌细胞，以响应绵羊红细胞，尽管在IL-2和少数T细胞存在时，对该因子的需求不太明显。该因子的明显含量约为40000 mol wt。该因子在正常的Con A Sn中被发现，该Sn通过IL-2依赖性T细胞的吸收而耗尽了IL-2，而在产生IL-2的T细胞杂杂瘤FS6-14.13的Con A刺激上清中不存在。这些结果表明，多种辅助因子控制着B细胞对抗原的反应，IL-2除了具有促进T细胞生长的活性外，还在B细胞反应中起直接作用。
A helper factor(s) distinct from interleukin 2 (IL-2) was shown to be present in the concanavalin A-stimulated supernatant of normal mouse spleen cells (normal Con A Sn). Spleen cells thoroughly depleted of T cells required both IL-2 and this factor to produce antibody-secreting cells in response to sheep erythrocytes, although in the presence of IL- 2 and a few T cells the requirement for the factor was less apparent. The factor had an apparent approximately 40,000 mol wt. The factor was found in normal Con A Sn that had been depleted of IL-2 by absorption with IL-2-dependent T cells and was absent from Con A-stimulated supernatants of the IL-2-producing T cell hybridoma, FS6-14.13. These results indicate that multiple helper factors control the B cell response to antigen and that IL-2, in addition to its T cell growth promoting activity, plays a direct role in B cell responses.