STRUCTURE OF THE HUMAN PHOSPHOGLYCERATE KINASE GENE AND THE INTRON-MEDIATED EVOLUTION AND DISPERSAL OF THE NUCLEOTIDE-BINDING DOMAIN
STRUCTURE OF THE HUMAN PHOSPHOGLYCERATE KINASE GENE AND THE INTRON-MEDIATED EVOLUTION AND DISPERSAL OF THE NUCLEOTIDE-BINDING DOMAIN
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DOI：
10.1073/pnas.82.20.6965
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ORKIN, SH
中科院分区：
文献类型：
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作者：
MICHELSON, AM;BLAKE, CCF;ORKIN, SH
克隆了在所有体细胞中表达的人X-连锁磷酸甘油酸激酶（PGK）基因，并测定了其结构。该基因被10个内含子中断，跨越23个内切酶。当投影在PGK蛋白分子的三维结构上时，剪接点位于已建立的肽结构域之间。特别地，内含子将ATP结合区的两个单核苷酸肽亚结构域分开，并且另外的内含子将这些亚结构域中的每一个在它们的特征性β-腺苷酸亚结构域之间分开。股。在乙醇脱氢酶和甘油醛-3-磷酸脱氢酶的二分NAD结合结构域中也发现了类似的相关性。此外，在每种情况下，核苷酸结合结构域通过至少一个内含子与催化结构域分开。这三种酶的结构相似区域中外显子组织的同源性表明，核苷酸结合结构域通过基因复制而进化，随后通过内含子介导的重组过程分散到不同的蛋白质中。
The human X-linked phosphoglycerate kinase (PGK) gene, which is expressed in all somatic cells, was cloned and its structure was determined. The gene is interrupted by 10 introns and spans 23 kilobases. When projected on the three-dimensional structure of the PGK protein molecule, splice junctions are located between established peptide domains. In particular, an intron separates the two mononucleotide subdomains of the ATP-binding region, and additional introns divide each of these subdomains between their characteristic .beta.-strands. Similar correlations are found in the bipartite NAD-binding domains of alcohol dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase. Furthermore, in each case the nucleotide-binding domain is separated from the catalytic domain by at least one intron. The homology of the exon organization in structurally similar regions of these three enzymes suggest that a nucleotide-binding domain evolved by gene duplication and was subsequently dispersed to different proteins through a process of intron-mediated recombination.