Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein.
Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein.
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DOI：
10.1016/j.virol.2017.02.008
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发表时间：
2017-05
期刊：
影响因子：
3.7
通讯作者：
Takimoto T
中科院分区：
文献类型：
--
作者：
Chaimayo C;Hayashi T;Underwood A;Hodges E;Takimoto T
甲型流感病毒含有八个单链、负义RNA片段，作为病毒核糖核蛋白（vRNP）形式的病毒基因组。在细胞核中的基因组复制期间，产生作为复制中间体的正义互补RNP（cRNP），其不并入子代病毒体中。为了分析选择性vRNP掺入子代病毒体的机制，我们使用链特异性qRT-PCR定量感染细胞的核和胞质组分中的vRNP和cRNP。出乎意料的是，我们发现cRNP也被输出到细胞质中。与vRNP不同，该输出是染色体区域维护1（CRM 1）独立的。虽然vRNP和cRNP都存在于胞质溶胶中，但病毒基质（M1）蛋白（病毒组装的关键调节因子）优先结合vRNP而不是cRNP。这些结果表明，甲型流感病毒通过在病毒组装期间与M1的特异性相互作用选择性地摄取胞质vRNP。
Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly.