Development of a lateral flow dipstick immunoassay for evaluation of folate levels in maize
Development of a lateral flow dipstick immunoassay for evaluation of folate levels in maize
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DOI：
10.1007/s00216-017-0504-6
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通讯作者：
王保民
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作者：
梁秋菊;姜凌;易尘;谭贵玉;张春义;王保民
Folates (vitamin B9) are essential for all organisms.as cofactors for one-carbon metabolism. However, measurement.of folates is technically complicated and time-consuming..In this study, we developed a dipstick immunoassay using a.folate-specific monoclonal antibody (mAb), allowing rapid.and low-cost detection of folates. The indicator range of the.dipstick for 5-formylterahydrofolate (5-CHO-THF), 5-.methyltetrahydrofolate (5-CH3-THF) and their polyglutamyl.forms was 100–200 ng mL−1; moreover, no cross-reactivity.was observed with tetrahydrofolate (THF) or 5,10-.methenyltetrahydrofolate (5,10-CH=THF) at 500 ng mL–1, or.with the folate precursors pterin-6-COOH, p-aminobenzoate.(pABA), and L-glutamate, or with the folate analogues methotrexate.and 10-formyltetrahydrofolate (10-CHO-THF) at up to.1000 ng mL−1. The dipstick immunoassay was tested in maize.seeds; the results classified the seeds into those with low, moderate,.and high levels of folates, and were in agreement with.those of liquid chromatography-mass spectrometry. Thus, we.conclude that the dipstick assay will provide a versatile tool to.facilitate large-scale screening of maize rich in folates.
