Surface functionalization of carbon-based sensors with biocompatible polymer to enable electrochemical measurement in protein-rich environment
Surface functionalization of carbon-based sensors with biocompatible polymer to enable electrochemical measurement in protein-rich environment
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DOI：
10.1016/j.snb.2020.127758
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发表时间：
2020-04
影响因子：
8.4
通讯作者：
T. Uen;K. Kushiro;H. Hibino;M. Takai
中科院分区：
文献类型：
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作者：
T. Uen;K. Kushiro;H. Hibino;M. Takai
电化学生物传感器已被广泛应用于阐明各种信息的药物重要的神经递质，如多巴胺（DA）。然而，电极的生物污染一直是在生理环境中进行的电化学测量的关键问题。在这项研究中，高生物相容性的聚合物刷制成的聚（2-甲基丙烯酰氧乙基磷酰胆碱）（PMPC），以修改碳基传感器，以防止生物污损。PMPC刷的厚度范围从15到30 nm的玻璃碳表面上成功地抑制蛋白质吸附在血清环境中，和聚合物改性保持稳定至少8小时，在这样的条件下。用循环伏安法（CV）测定，PMPC修饰的玻碳电极（GCE）对DA的检出限（LOD）低至1.8 μM，优于牛血清白蛋白（BSA）修饰的GCE（LOD为3.9 μM）。这项研究的结果为药物发现和药代动力学开辟了新的途径，使碳基生物传感器能够在富含蛋白质的环境中长时间准确灵敏地检测药物。
Electrochemical biosensors have been widely applied to elucidate various information on pharmaceutically important neurotransmitters such as dopamine (DA). However, the biofouling of electrodes has been a critical issue for electrochemical measurements performed in physiological environments. In this study, highly biocompatible polymer brush made of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was employed to modify carbon-based sensors to prevent biofouling. The PMPC brushes with thicknesses ranging from 15 to 30 nm on top of glassy carbon surfaces successfully suppressed protein adsorption in serum environments, and the polymer modification remained stable for at least 8 h in such conditions. The PMPC-modified glassy carbon electrode (GCE) achieved a limit of detection (LOD) as low as 1.8 μM for DA, as determined from cyclic voltammetry (CV), which exhibited better performance than the bovine serum albumin (BSA)-coated GCE with a LOD of 3.9 μM. The results from this study open new avenues for drug discovery and pharmacokinetics by enabling carbon-based biosensors to accurately and sensitively detect pharmaceuticals in protein-rich environments for an extensive period of time.