Effects of adrenalectomy on CCD: evidence for differential response of two cell types.
Effects of adrenalectomy on CCD: evidence for differential response of two cell types.
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肾上腺切除术对 CCD 的影响：两种细胞类型差异反应的证据。
DOI：
10.1152/ajprenal.1987.253.4.f742
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Sansom,S
中科院分区：
文献类型：
--
作者：
Muto,S;Giebisch,G;Sansom,S
用电生理和化学方法测定了正常和肾上腺切除（ADX）家兔皮质集合管（CCD）的钠、钾转运特性。对照组Na（JNa）和K（-JK）的净通量分别为5.7和3.2 pmol。mm-1。min ~（-1）和ADX的浓度分别为1.0和0.7pmol. mm-1。min-1，分别类似于电确定的速率。在单独的实验中，对肾上腺完整（第1组）、ADX（第2组）和用脱氧皮质酮处理的ADX兔（第3组）的细胞进行盲穿刺，可以鉴定两种不同的细胞类型，多数细胞（MA）和少数细胞（MI）。在所有组中，MA与MI的区别在于相对较高的基底外侧膜电位（-Vb），较低的顶膜电阻分数（FRa），以及顶膜和基底外侧膜K电导的存在。与第2组（-66.4 mV）相比，第1组和第3组的MA Vb（-82.4 mV）明显超极化。然而，第2组MI的Vb（-38.9 mV）与第1组和第3组MI的Vb（-36.2 mV）之间无显著差异。在第1组MA中，顶膜Na和K电导（GNaa、GKa）以及最大泵电流（Ipmax）的等效电路值分别为0.84和6.72 mS/cm 2以及46.7 microA/cm 2。第2组中的这些值显著较低（分别为0.28和1.52 mS/cm 2以及8.7 microA/cm 2）。它的结论是，两种细胞类型可以区分在CCD的电。MA具有与主细胞一致的性质，MI具有与插入细胞一致的性质。ADX导致PC的GNaa、GKa和Ipmax降低，导致INaa和IKa成比例降低。
Electrophysiological and chemical methods were used to determine the Na and K transport properties of the isolated cortical collecting duct (CCD) of control and adrenalectomized (ADX) rabbits. Net fluxes of Na (JNa) and K (-JK) in controls were 5.7 and 3.2 pmol . mm-1 . min-1 and in ADX were 1.0 and 0.7 pmol . mm-1 . min-1, respectively, similar to electrically determined rates. In separate experiments, blind impalement of cells from adrenal intact (group 1), ADX (group 2), and ADX rabbits treated with deoxycorticosterone (group 3) allowed identification of two distinct cell types, majority cells (MA) and minority cells (MI). In all groups, MA were distinguished from MI by a relatively high basolateral membrane potential (-Vb), low apical membrane fractional resistance (FRa), and presence of apical and basolateral membrane K conductances. Vb of MA (-82.4 mV) was significantly hyperpolarized in groups 1 and 3 combined, when compared with group 2 (-66.4 mV). However, there was no significant difference between Vb of MI in group 2 (-38.9 mV) and Vb of MI in groups 1 and 3 (-36.2 mV). In MA of group 1 equivalent circuit values of apical membrane Na and K conductances (GNaa, GKa) and maximum pump current (Ipmax) were 0.84 and 6.72 mS/cm2 and 46.7 microA/cm2, respectively. These values in group 2 were significantly lower (0.28 and 1.52 mS/cm2 and 8.7 microA/cm2, respectively). It is concluded that two cell types can be distinguished electrically in the CCD. MA have properties consistent with principal cells and MI have properties consistent with intercalated cells. ADX causes a decrease in GNaa, GKa, and Ipmax of PC that results in proportionate decreases in INaa and IKa.