Effects of antibodies against a fusion protein consisting of parts of cell surface protein antigen and glucosyltransferase of Streptococcus sobrinus on cell adhesion of mutans streptococci.
Effects of antibodies against a fusion protein consisting of parts of cell surface protein antigen and glucosyltransferase of Streptococcus sobrinus on cell adhesion of mutans streptococci.
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DOI：
10.1111/j.1399-302x.2007.00382.x
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发表时间：
2007-12
影响因子：
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通讯作者：
T. Kawato;Y. Yamashita;T. Katono;A. Kimura;M. Maeno
中科院分区：
文献类型：
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作者：
T. Kawato;Y. Yamashita;T. Katono;A. Kimura;M. Maeno
背景/目的远缘链球菌产生的细胞表面蛋白抗原（PAg）和葡萄糖基转移酶（GTF）被认为是该菌的主要定植因子。方法构建了一个融合基因，该融合基因编码由PAg的富含丙氨酸区（PAgA）和GTF-Ⅰ的葡聚糖结合区（GB）组成的蛋白，该蛋白催化S. sobrinus。从携带融合基因的大肠杆菌的细胞提取物中纯化融合蛋白PAgA-GB，并在兔中制备针对融合蛋白的抗体。结果在蔗糖存在下，抗PAgA-GB抗体能显著抑制S. sobrinus MT 8145和变形链球菌Xc对唾液包被羟基磷灰石微球的抑制作用。sobrinus对S.变种人在无蔗糖存在的情况下，抗PAgA-GB抗体显着抑制两种S的粘附。sobrinus和S.而对S. sobrinus比S.变种人用抗完整重组PAg蛋白（rPAg）的抗体观察到类似的结果，而同一抗体对S. sobrinus对S.变形细胞结论本实验结果表明，抗沙门氏菌的抗体具有良好的免疫原性。sobrinus GTF-1可能用于有效抑制S. sobrinus。而S. sobrinus可能主要不是作为获得性表膜的受体，其他细胞表面蛋白可能参与了S. sobrinus。
BACKGROUND/AIMS The cell surface protein antigen (PAg) and glucosyltransferases (GTFs) produced by Streptococcus sobrinus are considered to be major colonization factors of the organism. METHODS We constructed a fusion gene encoding a protein composed of the alanine-rich region of PAg (PAgA) and the glucan-binding domain (GB) of GTF-I, which catalyzes the synthesis of water-insoluble glucan in S. sobrinus. The fusion protein PAgA-GB was purified from cell extracts of Escherichia coli harboring the fusion gene, and antibodies against the fusion protein were prepared in rabbits. RESULTS In the presence of sucrose, the antibody against PAgA-GB significantly inhibited the adhesion of both S. sobrinus MT8145 and Streptococcus mutans Xc to saliva-coated hydroxyapatite beads, and the inhibitory effect on S. sobrinus was stronger than that on S. mutans. In the absence of sucrose, the antibody against PAgA-GB significantly inhibited the adhesion of both S. sobrinus and S. mutans, however the inhibitory effect on S. sobrinus was unexpectedly weaker than that on S. mutans. A similar result was observed with the antibody against the intact recombinant PAg protein (rPAg), while the same antibody reacted more strongly against S. sobrinus than against S. mutans cells. CONCLUSION Taken together, these results show that the antibody against S. sobrinus GTF-I may be useful for effective inhibition of the sucrose-dependent adhesion of S. sobrinus. However, PAg of S. sobrinus may not function primarily as a receptor for acquired pellicles, and other cell surface proteins may be involved in the sucrose-independent adhesion of S. sobrinus.