Micromagnetic-microfluidic blood cleansing device
Micromagnetic-microfluidic blood cleansing device
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DOI：
10.1039/b816986a
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Ingber, Donald E.
中科院分区：
文献类型：
--
作者：
Yung, Chong Wing;Fiering, Jason;Ingber, Donald E.
脓毒症是一种致命的疾病，由全身性微生物感染引起，通过血液传播，压倒身体的防御。目前的治疗方法往往是次优的，部分原因是它们不能完全消除病原体，因此也就是致命毒素的来源。在这里，我们描述了一种体外血液净化装置，以选择性地从受污染的血液中去除病原体，从而增强患者对抗生素治疗的反应。对免疫磁性微珠进行了修饰，以产生磁性调理素，用于净化流动的人类全血中的白色念珠菌真菌，这是脓毒症相关死亡的主要原因。微磁-微流体血液净化装置在垂直堆叠的通道上产生磁场梯度，以实现真菌与流动的全血的连续和高通量分离。该装置的多路复用版本包含四个平行通道，以20 mL/h的流速单程清除受污染血液中的真菌超过80%，比之前描述的原型微磁-微流体细胞分离系统快1000倍。这些结果提供了第一个原理证明，即多路复用微磁-微流体分离系统可以用于以与临床应用相关的速率和分离效率从流动的人类血液中清除病原体。
Sepsis is a lethal disease caused by a systemic microbial infection that spreads via the bloodstream to overwhelm the body's defenses. Current therapeutic approaches are often suboptimal, in part, because they do not fully eliminate the pathogen, and hence the source of deadly toxins. Here we describe an extracorporeal blood cleansing device to selectively remove pathogens from contaminated blood and thereby enhance the patient's response to antibiotic therapy. Immunomagnetic microbeads were modified to create magnetic opsonins that were used to cleanse flowing human whole blood of Candida albicans fungi, a leading cause of sepsis-related deaths. The micromagnetic-microfluidic blood cleansing device generates magnetic field gradients across vertically stacked channels to enable continuous and high throughput separation of fungi from flowing whole blood. A multiplexed version of the device containing four parallel channels achieved over 80% clearance of fungi from contaminated blood at a flow rate of 20 mL/h in a single pass, a rate 1000 times faster than a previously described prototype micromagnetic-microfluidic cell separation system. These results provide the first proof-of-principle that a multiplexed micromagnetic-microfluidic separation system can be used to cleanse pathogens from flowing human blood at a rate and separation efficiency that is relevant for clinical applications.