Sexual and developmental differences in peptides regulating growth hormone secretion in the rat.
Sexual and developmental differences in peptides regulating growth hormone secretion in the rat.
复制标题
调节大鼠生长激素分泌的肽的性别和发育差异。
DOI：
10.1159/000125237
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发表时间：
1989
影响因子：
4.1
通讯作者：
Martin,JB
中科院分区：
文献类型：
--
作者：
Gabriel,SM;Millard,WJ;Koenig,JI;Badger,TM;Russell,WE;Maiter,DM;Martin,JB
通过测量雄性和雌性大鼠的大鼠 GH 释放因子 (rGRF) 和生长抑素来研究下丘脑控制生长激素 (GH) 分泌的性别差异。与雌性大鼠相比，成年（90 日龄）雄性大鼠的中位隆起和中位隆起之外的下丘脑组织中的大鼠 GRF 样免疫反应性 (rGRF-IR) 较高。在 35 日龄大鼠的中位隆起中发现了类似的 rGRF-IR 含量模式。这种性别差异在 25 天至 35 天之间出现，在此期间，两性的胰岛素样生长因子 (IGF-1) 血清浓度和体重均增加。在较小程度上，与雄性大鼠相比，成年雌性大鼠中位隆起的生长抑素样免疫反应性（SLI）含量较高。整个下丘脑 rGRF-IR 和 SLI 含量仅受到成人性腺切除术或性腺类固醇治疗的中度影响。例如，雌激素增加了去势大鼠的 rGRF-IR 含量，但单独的睾丸切除术或睾丸切除术后使用睾酮并不影响 rGRF-IR 含量。此外，整个下丘脑 SLI 含量不受睾丸切除术或睾丸切除术随后睾酮或雌激素的影响。卵巢切除术后1个月，整个下丘脑片段中的rGRF-IR和SLI与性腺完整的男性中各自的含量相似。然而，卵巢切除术后使用雌激素或睾酮并不能恢复 rGRF-IR 含量，并且可以将 SLI 含量部分恢复至性腺完整女性的水平。
Sex differences in the hypothalamic control of growth hormone (GH) secretion were investigated by measuring rat GH-releasing factor (rGRF) and somatostatin in male and female rats. Rat GRF-like immunoreactivity (rGRF-IR) was higher in the median eminence and hypothalamic tissue outside of the median eminence of adult (90-day-old) male compared to female rats. A similiar pattern of rGRF-IR content was found in the median eminence of 35-day-old rats. This sex difference developed between days 25 and 35 of age, during which time serum concentrations of insulin-like growth factor (IGF-1) and body weight increased in both sexes. To a lesser extent, the content of somatostatin-like immunoreactivity (SLI) was higher in the median eminence of adult female rats compared to male rats. Whole hypothalamic rGRF-IR and SLI contents were influenced only moderately by adult gonadectomy or gonadal steroid treatments. For example, estrogen increased rGRF-IR content in castrated rats, but orchidectomy alone or orchidectomy followed by testosterone did not influence rGRF-IR content. Additionally, whole hypothalamic SLI content was unaffected by orchidectomy or orchidectomy followed by testosterone or estrogen. One month after ovariectomy, rGRF-IR and SLI in whole hypothalamic fragments were similar to their respective contents in gonad-intact males. However, ovariectomy followed by estrogen or testosterone did not restore rGRF-IR content and partially restored SLI content to levels seen in gonad-intact females.