Purkinje cell loss accompanies motor impairment in rats developing at altered gravity.
Purkinje cell loss accompanies motor impairment in rats developing at altered gravity.
复制标题
在改变重力条件下发育的大鼠中，浦肯野细胞损失伴随着运动障碍。
DOI：
10.1097/00001756-200512190-00014
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发表时间：
2005
期刊：
影响因子：
1.7
通讯作者：
Baxter,MarkG
中科院分区：
文献类型：
--
作者：
Sajdel-Sulkowska,ElizabethM;Nguon,Kosal;Sulkowski,ZacharyL;Rosen,GlennD;Baxter,MarkG
我们以前曾报道过，大鼠从妊娠第8天到出生后第21天暴露于改变的重力（1.65 g）会影响运动功能和小脑结构。本研究探讨是否在小脑质量的减少伴随着受损的性能在超重力暴露大鼠的旋转杆浦肯野细胞数量的减少。在出生后第21天，使用体视学分析确定浦肯野细胞的总数，该体视学分析应用于随后包埋在火棉胶中的多聚甲醛固定的小脑样品。总浦肯野细胞数量减少17.7- 25.3%。这些结果表明，暴露于改变重力浦肯野细胞出生过程中可能会影响其增殖，导致浦肯野细胞数量减少，这反过来又会导致运动障碍。
We have previously reported that the developmental exposure of rats to altered gravity (1.65 g) from gestational day 8 to postnatal day 21 impacts motor functions and cerebellar structure. The present study examined whether the decrease in cerebellar mass accompanied by impaired performance on a rotorod in hypergravity-exposed rats was related to a decrease in Purkinje cell number. The total number of Purkinje cells was determined on postnatal day 21 using a stereological analysis applied to paraformaldehyde-fixed cerebellar samples subsequently embedded in celloidin. Total Purkinje cell number was decreased by 17.7–25.3%. These results imply that exposure to altered gravity during Purkinje cell birth may affect their proliferation, resulting in a decrease in Purkinje cell number, which, in turn, leads to motor impairment.