Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets
Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets
复制标题
DOI：
10.1079/bjn20061733
复制
发表时间：
2006-05-01
影响因子：
3.6
通讯作者：
Liu, YJ
中科院分区：
文献类型：
--
作者：
Du, ZY;Clouet, P;Liu, YJ
高脂饲料对某些肉食性鱼类的生长具有有利的影响，这是由于脂质具有节省蛋白质的作用，但高脂饲料也会对肉质产生一些负面影响。因此，本研究的目的是确定富脂饲料对草鱼（Ctenopharyngodon idella）幼鱼的生长和可能的脂质代谢的影响。在再循环系统中，将含有2%、6%或10%的猪油、玉米油和鱼油（1：1：1，按重量计）的混合物的三种等氮饮食应用于鱼8周。数据显示，饲喂脂质水平增加的饲料会导致摄食量降低、生长和饲料效率降低以及肠系膜脂肪组织重量增加。同时，脂蛋白合成的改变和更高水平的脂质过氧化在血液中是明显的。在肝脏、肌肉和肠系膜脂肪组织中，α-亚麻酸和DHA的百分比显著增加或有增加趋势。对肝脏进行的生化活性测量表明，随着膳食脂质水平的增加，线粒体和过氧化物酶体脂肪酸氧化能力都有所下降，这可能至少部分地导致α-亚麻酸和DHA在细胞膜构建中更活跃的特定积累。总体而言，草鱼难以充分利用过量的饲料脂肪，尤其是富含易过氧化的n-3 PUFA时。
High-fat diets may have favourable effects on growth of some carnivorous fish because of the protein-sparing effect of lipids, but high-fat diets also exert some negative impacts on flesh quality. The goal of the study was therefore to determine the effects of fat-enriched diets in juvenile grass carp (Ctenopharyngodon idella) as a typical herbivorous fish on growth and possible lipid metabolism alterations. Three isonitrogenous diets containing 2, 6 or 10 % of a mixture of lard, maize oil and fish oil (1:1:1, by weight) were applied to fish for 8 weeks in a recirculation system. Data show that feeding diets with increasing lipid levels resulted in lowered feed intake, decreased growth and feed efficiency, and increased mesenteric fat tissue weight. Concomitantly, alteration of lipoprotein synthesis and greater level of lipid peroxidation were apparent in blood. In liver, muscle and mesenteric fat tissue, the percentages of alpha-linolenic acid and DHA were significantly increased or tended to increase with higher dietary lipid levels. Biochemical activity measurements performed on liver showed that, with the increase in dietary lipid level, there was a decrease in both mitochondrial and peroxisomal fatty acid oxidation capacities, which might contribute, at least in part, to the specific accumulation of alpha-linolenic acid and DHA into cells more active in membrane building. On the whole, grass carp have difficulty in energetically utilising excess dietary fat, especially when enriched in n-3 PUFA that are susceptible to peroxidation.