COMPARISON OF THE ANTIPROLIFERATIVE EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA,N,N-DIMETHYLFORMAMIDE AND RETINOIC ACID ON A HUMAN-COLON CARCINOMA CELL-LINE
COMPARISON OF THE ANTIPROLIFERATIVE EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA,N,N-DIMETHYLFORMAMIDE AND RETINOIC ACID ON A HUMAN-COLON CARCINOMA CELL-LINE
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DOI：
10.1016/0304-3835(88)90014-6
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发表时间：
1988-06-15
期刊：
影响因子：
9.7
通讯作者：
BRATTAIN, MG
中科院分区：
文献类型：
--
作者：
HOOSEIN, NM;BRATTAIN, DE;BRATTAIN, MG
在这项研究中，我们比较了转化生长因子-.β的抗增殖作用。 (TGF-β)、N,N-二甲基甲酰胺(DMF)和视黄酸(RA)对中分化结肠癌细胞系(JVC)的作用。 TGF-β、DMF和RA抑制JVC细胞的贴壁依赖性生长并诱导形态变化。获得40pM TGF-β、0.5％DMF和5nM RA的生长抑制的EC50值。此外，所有三种药物均以时间和剂量依赖性方式增强细胞纤连蛋白水平。所有三种药物对细胞增殖的抑制以及纤连蛋白的诱导都是可逆的。任何两种药剂以次优剂量的组合同时添加到 JVC 细胞中会产生附加的生长抑制反应。这些数据表明TGF-β的作用。在该结肠癌细胞系中，与两种分化促进剂DMF和RA相似。
In this study we have compared the anti-proliferative effects of transforming growth factor-.beta. (TGF-.beta.), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-.beta., DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC50 values of 40 pM TGF-.beta., 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-.beta. in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.