RETROGRADE REACTION IN MOTONEURON DENDRITES FOLLOWING VENTRAL ROOT SECTION IN THE FROG
RETROGRADE REACTION IN MOTONEURON DENDRITES FOLLOWING VENTRAL ROOT SECTION IN THE FROG
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DOI：
10.1002/cne.901090205
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发表时间：
1958-01-01
影响因子：
2.5
通讯作者：
CHACKO, LW
中科院分区：
文献类型：
--
作者：
CERF, JA;CHACKO, LW
对林蛙脊髓运动神经元的逆行反应进行了研究。单侧脊髓前根切断后8~11d取脊髓腰段(第8、9、10、有时为第11根)，切片按尼氏硫代蓝染色、Bodian‘S蛋白浸渍、Gomori’S酸性磷酸酶组织化学方法检测。切开轴突后，在手术侧运动神经元周围证实了众所周知的细胞反应：染色溶解，对银的亲和力降低，神经纤维解体，酸性磷酸酶活性升高。在运动神经元树突中也发现了类似的变化。两栖动物脊髓的树突的特征模式，即长的扩张，大量分支穿过腹外侧柱的白质，并在软脑膜下形成致密的边缘神经丛，这使得可以识别出逆行效应延伸到运动神经元树突的整个长度。树突近端尼氏物质的溶解和神经纤维的解体与亲本细胞体的染色溶解有关。在包括髓周丛在内的整个树突分支中，观察到对银的亲和力降低和酸性磷酸酶活性升高。其他实验表明，早在轴突切断后20小时，腹外侧柱中酸性磷酸酶反应就增加了。结果表明，树枝晶广泛参与了逆反应的各个过程。
Retrograde reaction was studied in spinal motoneurons of Ranacatesbeiana. The lumbar segment of the spinal cord was removed 8-11 days after unilateral ventral root amputation (8th, 9th, 10th, sometimes 11th root), and sections were treated according to the following techniques Nissl thionin-blue stain, Bodian''s protargol impregnation, Gomori''s method for histochemical detection of acid phosphatase. Well-known cellular reactions following axonal section were confirmed in the motoneuron perikarya on the operated side: chromatolysis, decreased affinity for silver and neurofibrillar disintegration, heightened acid phosphatase activity. Similar alterations were traced further in the motoneuron dendrites. The characteristic pattern of dendritic processes in amphibian spinal cord, i.e. long expansions branching profusely through the white matter in the ventrolateral column and contributing to a dense marginal plexus under the pia, permitted the identification of retrograde effects extending to the entire length of the motoneuron dendrites. Dissolution of Nissl substance and neurofibrillar disintegration in the proximal shaft of the dendrites were found associated with chromatolysis in the parent cell-bodies. Decreased affinity for silver and heightened acid phosphatase activity were observed in the whole dendritic arborization, including the perimedullary plexus. Other experiments revealed an increased acid phosphatase reaction in the ventro-lateral column as early as 20 hours after axotomy. The results suggest an extensive participation of dendrites in various processes of the retrograde reaction.