Neuromotor Activity, Anxiety and Cognitive Function in the In Vivo Model of Alimentary Hyperlipidemia and Obesity
Neuromotor Activity, Anxiety and Cognitive Function in the In Vivo Model of Alimentary Hyperlipidemia and Obesity
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DOI：
10.1007/s10517-017-3732-z
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发表时间：
2017-05-01
影响因子：
0.7
通讯作者：
Mazo, V. K.
中科院分区：
文献类型：
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作者：
Apryatin, S. A.;Sidorova, Yu. S.;Mazo, V. K.
采用大鼠和小鼠体内营养性高脂血症模型，研究了表征营养依赖性疾病病理过程具体特征的行为指标。对照组的大鼠和小鼠接受平衡半合成饮食63天;实验组的动物接受高脂肪含量（30%干重）的饮食、用果糖溶液代替水的平衡或高脂肪饮食、富含胆固醇的平衡饮食（0.5%干重）或富含胆固醇的平衡饮食与果糖溶液。在实验期间，每天监测动物消耗的食物质量。在实验的第33天和第54天通过前爪抓握强度评估肌张力。在第36天和第57天在高架十字迷宫中测试焦虑。在第39、40和61天通过条件被动回避反射评估行为和记忆。在第54天，用果糖平衡饮食喂养的大鼠和接受类似饮食补充果糖和胆固醇的小鼠的肌肉张力显着增加。在第二次测试（第57天）中，喂食高脂饮食的大鼠的焦虑显著降低，喂食高脂饮食和高脂饮食与果糖的小鼠的焦虑增加。在第二个测试中，饮食中额外的胆固醇是显着改善两个物种的短期和长期记忆的因素。在小鼠中，与大鼠相反，添加果糖，包括与高脂肪饮食相结合，显着恶化短期和长期记忆。因此，导致大鼠和小鼠消化道血脂异常发展的饮食因素可显著影响神经运动活动、焦虑水平和认知功能的指数，这些变化的性质和方向在很大程度上具有种属特异性。
Behavioral indicators characterizing specific features of the pathological process of alimentary-dependent diseases were studied using in vivo model of alimentary hyperlipidemia in rats and mice. Rats and mice of the control groups received balanced semisynthetic diet for 63 days; animals of the experimental groups received a diet with high fat content (30% dry weight), balanced or high-fat diet with fructose solution instead of water, balanced cholesterol-enriched diet (0.5% dry weight), or balanced cholesterol-enriched diet with fructose solution. During the experiment, the mass of food, consumed by the animals, was monitored daily. Muscle tone was assessed by the front paw grip strength on days 33 and 54 of the experiment. Anxiety was tested in the elevated plus maze on days 36 and 57. Behavior and memory were assessed by conditioned passive avoidance reflex on days 39, 40, and 61. A significant increase in muscle tone was revealed on day 54 in rats fed with a balanced diet with fructose, and in mice, that received a similar diet, supplemented with fructose and cholesterol. Anxiety in the second test (day 57) was significantly decreased in rats fed high-fat diet and increased in mice fed high fat diet and high fat diet with fructose. In the second test, additional amount of cholesterol in the diet was the factor that significantly improved both short-term and long-term memory in both species. In mice, in contrast to rats, addition of fructose, including combination with high-fat diet, significantly worsened short-term and long-term memory. Thus, dietary factors, contributing to alimentary dyslipidemia development in rats and mice, can significantly affect the indices of neuromotor activity, anxiety level and cognitive functions, and the nature and direction of these changes are largely species-specific.