Sequential cytologic study of the development of squamous cell carcinoma induced in subcutaneously implanted bronchial autograft of dog.
Sequential cytologic study of the development of squamous cell carcinoma induced in subcutaneously implanted bronchial autograft of dog.
复制标题
狗皮下植入自体支气管移植物诱导鳞状细胞癌发生的序贯细胞学研究。
作者：
N. Kobayashi;M. Kanisawa;T. Okamoto;M. Okita;H. Katsuki
对犬移植支气管腔内致癌多环烃暴露引起的鳞状上皮化生和鳞状细胞癌的发展进行细胞学观察，并与同期活检标本的组织学和超微结构观察结果相关联。细胞学标本显示，在浸润性鳞状细胞癌发展之前，鳞状化生细胞的轻度非典型性进展到中度非典型性，以及显著的非典型性。活检标本获得的组织学结果显示与这些细胞学改变相对应。癌前阶段还可见鳞状上皮异型增生和原位癌样改变。上皮细胞的超微结构改变，如细胞间隙增宽，张力丝增多，核增大，凹陷较深，核仁突出，微分离，是癌前病变的特征性改变。结论：该细胞学方法反映了组织学和超微结构的变化，是比较研究人支气管癌前病变及其早期癌变的有用的实验工具。
Squamous cell metaplasias and the development of squamous cell carcinomas caused by carcinogenic polycyclic hydrocarbon exposure of the lumen of grafted dog bronchi were followed by cytology, and were correlated to histologically and ultrastructurally observed changes seen in the biopsy specimens at the same time periods. Cytologic specimens showed a progression from mild atypia of squamous metaplastic cells, to moderate atypia, and to marked atypia before the development of invasive squamous cell carcinoma. Histology obtained by the biopsy specimens revealed findings corresponding to these cytologic changes. In the premalignant phase, squamous dysplasia and carcinoma in situ-like changes were also seen. Ultrastructural findings of the epithelium, such as widened intercellular space, increasing tonofilaments, enlarged nuclei with deep indentations, and prominent nucleoli with microsegregation were characteristic changes in the preneoplastic stages. It was concluded that this method of cytology, reflecting histologic and ultrastructural changes, is a useful experimental tool for the comparative study of premalignant lesions in the human bronchus and their early neoplastic stages.