Semisynthesis of erythropoietin analogue having three oligosaccharides
Semisynthesis of erythropoietin analogue having three oligosaccharides
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具有三种寡糖的促红细胞生成素类似物的半合成
DOI：
10.1080/07328303.2011.604570
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Y.Kajihara
中科院分区：
文献类型：
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作者：
K.Hirano;M.Izumi;D.Macmillan;K.Tezuka;T.Tsuji;Y.Kajihara
合成了具有三种复合型双天线唾液酸寡糖的促红细胞生成素(EPO)类似物。将化学合成的EPO1-32氨基酸残基对应的三糖基肽α硫酯与化学合成的EPO33-16 6氨基酸残基对应的多肽进行天然化学连接，制得全长EPO糖肽。原核表达。全长糖多肽的氧化折叠提供了具有生物活性的EPO类似物。
An erythropoietin (EPO) analog having three complex-type biantennary sialyloligosaccharides was prepared. The full-length EPO glycopeptide was prepared through native chemical ligation of a triglycosylated peptide-αthioester corresponding to EPO 1–32 amino acid residues, which was synthesized chemically, and a polypeptide corresponding to EPO 33–166 amino acid residues, which was prepared byE. coliexpression. Oxidative folding of the full-length glycopolypeptide afforded the biologically active EPO analog.