The analysis of the complete mitochondrial genome of Lecanicillium muscarium (synonym Verticillium lecanii) suggests a minimum common gene organization in mtDNAs of Sordariomycetes:: phylogenetic implications
The analysis of the complete mitochondrial genome of Lecanicillium muscarium (synonym Verticillium lecanii) suggests a minimum common gene organization in mtDNAs of Sordariomycetes:: phylogenetic implications
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DOI：
10.1016/j.fgb.2004.07.003
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发表时间：
2004-10-01
影响因子：
3
通讯作者：
Typas, MA
中科院分区：
文献类型：
--
作者：
Kouvelis, VN;Ghikas, DV;Typas, MA
分析了昆虫病原真菌蝇蜡蚧轮枝菌（Verticilliumlecanii）的线粒体基因组（mtDNA），其总长度为24，499-bp。它是盘菌亚门中已知的最小的线粒体基因组，基因组结构非常紧凑，在其大的核糖体RNA（rnl）基因中只有一个Ⅰ组内含子。它包含编码氧化磷酸化相关蛋白质的14个典型基因，两个rRNA基因，一个内含子ORE编码可能的核糖体蛋白（rps），以及一组25个tRNA基因，其识别除丙氨酸和半胱氨酸外的所有氨基酸的密码子。所有的基因都是从同一条DNA链转录的。与所有可用的完整的真菌mtDNA的基因顺序比较-代表所有四门包括-揭示了一些特征的共同特征，如不间断的基因对，重叠的基因，和非常可变的基因间区域，这都可以被利用的研究真菌线粒体基因组。此外，所有已知的壳孢菌的mtDNA基因序列中，可以检测到一个最小的共同的，在两个单位，即nad 1-nad 4-atp 8-atp 6和rns-cox 3-rnl，可以扩展到肉座菌，nad 4L-nad 5-cob-cox 1-nad 1-nad 4-atp 8-atp 6和rns-cox 3-rnl nad 2-nad 3。将所有真菌mtDNA必需蛋白编码基因作为一个单位进行系统发育分析，清楚地证明了小基因组（mtDNA）比单基因比较的优越性。(C)2004年爱思唯尔公司All rights reserved.
The mitochondrial genome (mtDNA) of the entomopathogenic fungus Lecanicillium muscarium (synonym Verticillium lecanii) with a total size of 24,499-bp has been analyzed. So far, it is the smallest known mitochondrial genome among Pezizomycotina, with an extremely compact gene organization and only one group-I intron in its large ribosomal RNA (rnl) gene. It contains the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, one intronic ORE coding for a possible ribosomal protein (rps), and a set of 25 tRNA genes which recognize codons for all amino acids, except alanine and cysteine. All genes are transcribed from the same DNA strand. Gene order comparison with all available complete fungal mtDNAs-representatives of all four Phyla are included-revealed some characteristic common features like uninterrupted gene pairs, overlapping genes, and extremely variable intergenic regions, that can all be exploited for the study of fungal mitochondrial genomes. Moreover, a minimum common mtDNA gene order could be detected, in two units, for all known Sordariomycetes namely nad1-nad4-atp8-atp6 and rns-cox3-rnl, which can be extended in Hypocreales, to nad4L-nad5-cob-cox1-nad1-nad4-atp8-atp6 and rns-cox3-rnl nad2-nad3, respectively. Phylogenetic analysis of all fungal mtDNA essential protein-coding genes as one unit, clearly demonstrated the superiority of small genome (mtDNA) over single gene comparisons. (C) 2004 Elsevier Inc. All rights reserved.