DIFFERENTIAL EXPRESSION OF CAP AND CAP2 IN ADULT-RAT TISSUES
DIFFERENTIAL EXPRESSION OF CAP AND CAP2 IN ADULT-RAT TISSUES
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DOI：
10.1016/0378-1119(95)00522-8
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发表时间：
1995-11-20
期刊：
影响因子：
3.5
通讯作者：
YOUNG, D
中科院分区：
文献类型：
--
作者：
SWISTON, J;HUBBERSTEY, A;YOUNG, D
我们以前曾报道过人类CAP和CAP2基因的鉴定，它们编码与酵母腺酰环化酶(CyR)相关的CAP蛋白相关的蛋白。大鼠CAP同源基因MCH1也已被克隆。我们已经克隆了一个编码大鼠CAP2同源基因的cDNA。大鼠CAP/MCH1和CAP2的同源性为63%。采用逆转录-聚合酶链式反应(RT-PCR)方法，检测了成年大鼠不同组织中CAP/MCH1和CAP2基因的表达水平。我们的结果显示这两个基因的表达模式有很大的不同。与以前的报告一致，我们在所有被检查的组织中检测到CAP/MCH1 mRNA；然而，不同组织之间的水平有很大差异。特别是，我们发现CAP/MCH1在脾、睾丸和肺中的表达水平相对较高，在脑、肾脏、肝脏和小肠中的表达水平中等，在心脏、骨骼肌和皮肤中的表达水平明显较低。我们还用针对人CAP：：GST融合蛋白的多克隆抗体进行免疫印迹，研究了大鼠组织中CAP/MCH1的水平。总的来说，我们发现CAP/MCH1的mRNA水平反映了在不同组织中发现的CAP/MCH1的量。相反，CAP2转录本在睾丸中的表达水平相对较高，在脑、心脏和骨骼肌中处于中等水平，在肺、皮肤、肾脏和小肠中的表达水平较低，在肝脏和脾中检测不到。CAP/MCH1和CAP2的序列和表达模式之间的差异是显著的，表明这些蛋白具有不同的功能。
We previously reported the identification of the human CAP and CAP2 genes which encode proteins related to the yeast adenylyl cyclase (CYR)-associated CAP protein. The rat CAP homolog, MCH1, has also been previously cloned. We have cloned a cDNA encoding the rat homolog of CAP2. Rat CAP/MCH1 and CAP2 are 63% identical to each other. Using the reverse transcription-polymerase chain reaction (RT-PCR) method, we have examined CAP/MCH1 and CAP2 mRNA levels in various adult rat tissues. Our results show a dramatic difference in the pattern of expression of these two genes. Consistent with previous reports, we detected CAP/MCH1 mRNA in all tissues examined; however, levels vary substantially between tissues. In particular, we found that CAP/MCH1 mRNA are present at relatively high levels in spleen, testes and lung, at moderate levels in brain, kidney, liver and small intestine, and at significantly lower levels in heart, skeletal muscle and skin. We have also investigated the levels of CAP/MCH1 in rat tissues by immunoblotting with a polyclonal antibody raised against a human CAP::GST fusion protein. In general, we find that the CAP/MCH1 mRNA levels reflect the amount of CAP/MCH1 found in different tissues. In contrast, CAP2 transcripts were present at relatively high levels in testes, at moderate levels in brain, heart and skeletal muscle, at lower levels in lung, skin, kidney and small intestine, and were undetectable in liver or spleen. The differences between the sequences and expression patterns of CAP/MCH1 and CAP2 are significant and suggest that these proteins have distinct functional roles.