Impact of Artificial Sputum Medium Formulation on Pseudomonas aeruginosa Secondary Metabolite Production.
Impact of Artificial Sputum Medium Formulation on Pseudomonas aeruginosa Secondary Metabolite Production.
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DOI：
10.1128/jb.00250-21
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发表时间：
2021-10-12
影响因子：
3.2
通讯作者：
Phelan VV
中科院分区：
文献类型：
--
作者：
Neve RL;Carrillo BD;Phelan VV
正在开发体外培养基，以了解宿主位点特异性营养成分如何影响微生物致病性和生态。为了模拟囊性纤维化 (CF) 肺环境，人们创建了多种人工痰介质 (ASM)。然而，这些 ASM 的组成在关键营养素的浓度上有所不同，包括氨基酸、脂质、DNA 和粘蛋白。在这项工作中，我们使用基于特征的分子网络 (FBMN) 对铜绿假单胞菌进行比较代谢组学，铜绿假单胞菌是感染 CF 患者肺部的主要机会病原体，在九种不同的 ASM 中培养。我们发现添加到培养基中的粘蛋白中的芳香族氨基酸和铁的浓度导致铜绿假单胞菌毒力相关的次级代谢产物的产生差异。重要性旨在复制体内感染环境的不同培养基配方含有不同的营养物质，这会影响实验结果的解释。在化学成分明确的培养基中加入未定义的成分，例如商业猪胃粘蛋白（PGM），可能会以意想不到的方式改变培养基的营养成分并影响实验结果。
In vitro culture media are being developed to understand how host site-specific nutrient profiles influence microbial pathogenicity and ecology. To mimic the cystic fibrosis (CF) lung environment, a variety of artificial sputum media (ASM) have been created. However, the composition of these ASM vary in the concentration of key nutrients, including amino acids, lipids, DNA, and mucin. In this work, we used feature-based molecular networking (FBMN) to perform comparative metabolomics of Pseudomonas aeruginosa, the predominant opportunistic pathogen infecting the lungs of people with CF, cultured in nine different ASM. We found that the concentration of aromatic amino acids and iron from mucin added to the media contributes to differences in the production of P. aeruginosa virulence-associated secondary metabolites. IMPORTANCE Different media formulations aiming to replicate in vivo infection environments contain different nutrients, which affects interpretation of experimental results. Inclusion of undefined components, such as commercial porcine gastric mucin (PGM), in an otherwise chemically defined medium can alter the nutrient content of the medium in unexpected ways and influence experimental outcomes.