Epibolin: a protein of human plasma that supports epithelial cell movement.
Epibolin: a protein of human plasma that supports epithelial cell movement.
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Epibolin：人血浆中的一种蛋白质，支持上皮细胞运动。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
K. Stenn
中科院分区：
文献类型：
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作者：
K. Stenn
早期的研究表明，在哺乳动物血清和血浆中存在支持表皮（上皮）细胞运动的特定活性。该活性被证明是不可透析的和热不稳定的。在这里报道的研究中，使用标准的生化程序--即，硫酸铵分级分离、离子交换和凝胶过滤层析、等电沉淀和制备聚丙烯酰胺凝胶电泳--我们已经从人血浆中纯化了一种支持表皮细胞运动的因子。该因子在圆盘凝胶电泳上以明显的单一条带移动，对应于约65，000 +/-3，000道尔顿的糖基化单链蛋白。纯化的部分是必要的和足够的解离的表皮细胞，（ii）从皮肤外植体的上皮片的生长，和（iii）epiboly，上皮片移动在一个浮动的皮肤外植体。纯化的级分在1-2微克/ml生长培养基的浓度下是有活性的。它被胰蛋白酶破坏，其活性被第二个尚未纯化的血浆部分增强10倍以上。这些研究支持这样的观点，即血浆中的单一蛋白支持表皮细胞运动，并且该蛋白可能在伤口闭合中起重要作用。因为它支持epiboly，最生物相关的测定，它已被命名为epibolin。
Earlier studies suggested that there is a specific activity in mammalian serum and plasma that supports epidermal (epithelial) cell movement. This activity was shown to be nondialyzable and heat labile. In the studies reported here, using standard biochemical procedures--i.e., ammonium sulfate fractionation, ion-exchange and gel filtration chromatography, isoelectric precipitation, and preparative polyacrylamide gel electrophoresis--we have purified a factor from human plasma that supports epidermal cell movement. The factor travels as an apparent single band on disc gel electrophoresis and corresponds to a glycosylated single-chain protein of approximately 65,000 +/- 3,000 daltons. The purified fraction is necessary and sufficient for dissociated epidermal cells, (ii) outgrowth of epithelial sheets from skin explants, and (iii) epiboly, epithelial sheet movement over a floating skin explant. The purified fraction is active at a concentration of 1-2 micrograms/ml of growth medium. It is destroyed by trypsin and its activity is augmented more than 10-fold by a second, as yet unpurified, fraction of plasma. These studies support the notion that a single protein of plasma supports epidermal cell movement and that this protein may play an important role in wound closure. Because it supports epiboly, the most biologically relevant of the assays, it has been named epibolin.