Mass spectrometric analysis of body fluids for biomarker discovery.
Mass spectrometric analysis of body fluids for biomarker discovery.
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用于发现生物标志物的体液质谱分析。
DOI：
10.1007/978-1-59745-562-6_18
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Coon,JoshuaJ
中科院分区：
文献类型：
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作者：
Good,DavidM;Coon,JoshuaJ
基于细胞外蛋白质和多肽将提供特定器官甚至整个生物体的生理状态信息的概念，用于生物标志物发现的生物体液蛋白质组学分析近年来得到了迅速发展。尽管多项研究已成功使用质谱分析技术来测定样品中的蛋白质，但对天然存在的物种进行检查仍然很困难，大多数分析都使用自下而上的方法。我们应用了一种新的碎片方法，即电子转移解离（ETD）来解决这个问题。我们之前已经阐述了结合使用互补断裂技术、ETD 和碰撞激活解离来分析生物液体中天然存在的蛋白质和多肽时对光谱质量和总体鉴定的好处。
Hinging on the concept that extracellular proteins and polypeptides will provide information on the physiological state of specific organs, or even entire organisms, proteomic analysis of biological fluids for biomarker discovery has seen rapid expansion in recent years. Although multiple studies have had success using mass spectrometric analytical techniques for determination of proteins within a sample, inspection of naturally occurring species has been difficult, with most analyses using bottom-up methodology. We have applied a new fragmentation method, electron transfer dissociation (ETD), to this problem. We have previously illustrated the benefits to spectral quality and total identifications when using a combination of the complementary fragmentation techniques, ETD, and collision-activated dissociation, for analysis of naturally occurring proteins and polypeptides within biological fluids.