Human monoclonal antibodies to a genus-specific chlamydial antigen, produced by EBV-transformed B cells.
Human monoclonal antibodies to a genus-specific chlamydial antigen, produced by EBV-transformed B cells.
复制标题
针对属特异性衣原体抗原的人单克隆抗体，由 EBV 转化的 B 细胞产生。
DOI：
10.4049/jimmunol.130.6.2899
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发表时间：
1983
影响因子：
4.4
通讯作者：
G. Klein
中科院分区：
文献类型：
--
作者：
A. Rosén;K. Persson;G. Klein
稳定的B细胞系产生的人单克隆抗体衣原体建立从输卵管炎患者在早期恢复期。通过Epstein巴尔病毒（EBV）转化使抗体产生细胞永生化。通过逐步微量滴定板克隆程序富集特异性抗体分泌克隆。选择的B细胞克隆在连续培养中显示稳定的抗体产生超过1年。通过对一组衣原体参考菌株的微量免疫荧光（微量IF）试验证明了血清学特异性。抗体为IgG1亚类，并且可以证明一个克隆的补体固定。对大量其他细菌无交叉反应性。单克隆抗体针对衣原体生物体的常见属特异性表面抗原。感染的麦考伊细胞显示出明亮的、间断的荧光，周围环绕着包涵体膜。与常规抗血清相比，该单克隆抗体显示出更清晰的荧光模式，背景非常低。
Stable B cell lines producing human monoclonal antibodies to Chlamydia were established from salpingitis patients in the early convalescence phase. The antibody-producing cells were immortalized by Epstein Barr virus (EBV) transformation. Specific antibody-secreting clones were enriched by a stepwise microtiter plate cloning procedure. The selected B cell clones showed stable antibody production for more than 1 yr in continuous culture. Serologic specificity was demonstrated by micro-immunofluorescence (micro-IF) tests against a panel of Chlamydia reference strains. The antibodies were of the IgG1 subclass, and complement fixation could be demonstrated for one clone. There was no cross-reactivity against a large number of other bacteria. The monoclonal antibodies are directed against a common genus-specific surface antigen of the Chlamydia organism. Infected McCoy cells showed a brilliant, punctuated fluorescence surrounded by an inclusion membrane. Compared with conventional antisera, the monoclonal antibodies showed a clearer fluorescence pattern with very low background.