Polymer-induced bundling of F actin and the depletion force -: art. no. 051907
Polymer-induced bundling of F actin and the depletion force -: art. no. 051907
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DOI：
10.1103/physreve.69.051907
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发表时间：
2004-05-01
影响因子：
2.4
通讯作者：
Tang, JX
中科院分区：
文献类型：
--
作者：
Hosek, M;Tang, JX
当惰性聚合物聚乙二醇 (PEG) 浓度超过临界起始值 C-o 时，会在 F 肌动蛋白溶液中诱导“成束”现象。在 PEG 分子量和离子强度的有限范围内，C-o 可以表示为这两个变量的函数。该过程是可逆的，但在束的溶解中也观察到滞后现象，其中离子强度有很大影响。额外的肌动蛋白丝能够连接先前形成的肌动蛋白丝。 PEG 聚合物不并入肌动蛋白束中。将 Asakura-Oosawa 耗尽力、库仑斥力和范德华势的估计结合起来，以解释集束效应和滞后现象。提出了关于束尺寸的明显限制的猜想。
The inert polymer polyethylene glycol (PEG) induces a "bundling" phenomenon in F actin solutions when its concentration exceeds a critical onset value C-o. Over a limited range of PEG molecular weight and ionic strength, C-o can be expressed as a function of these two variables. The process is reversible, but hysteresis is also observed in the dissolution of the bundles, with ionic strength having a large influence. Additional actin filaments are able to join the previously formed bundles. PEG polymers are not incorporated into the actin bundles. Estimates of the Asakura-Oosawa depletion force, Coulomb repulsion, and van der Waals potential are combined in order to explain the bundling effect and hysteresis. Conjectures are presented concerning the apparent limit in bundle size.