Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing
Biological effects of stored platelet-rich plasma eye-drops in corneal wound healing
复制标题
储存的富血小板血浆滴眼液对角膜伤口愈合的生物学效应
DOI：
10.1136/bjo-2022-322068
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发表时间：
2022
影响因子：
4.1
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Okumura Yuichi;Inomata Takenori;Fujimoto Keiichi;Fujio Kenta;Zhu Jun;Yanagawa Ai;Shokirova Hurramhon;Saita Yoshitomo;Kobayashi Yohei;Nagao Masahi;et al.
背景/目的本研究旨在评估与自体血清（AS）滴眼液相比，富血小板血浆（PRP）滴眼液用于角膜上皮伤口愈合的有效性和无菌性。在长达4周的储存期间评估血小板和白细胞计数以及转化生长因子（TGF）-β1、表皮生长因子（EGF）和纤连蛋白水平。通过培养4周储存后样品评估无菌性。PRP，AS和磷酸盐缓冲盐水（PBS）滴眼液的功效进行了比较，使用角膜上皮伤口愈合assaysin vitroandin vivo和监测伤口面积在显微镜下每3 hours.ResultsHigher血小板和较低的白细胞计数被认为是在PRP比在全血中的一天的准备。保存后PRP中TGF-β1、EGF和FN水平显著高于AS滴眼液，在体外和体内，与PBS相比，PRP滴眼液可显著促进角膜上皮伤口愈合。此外，PRP滴眼液存储4周显着促进角膜伤口愈合相比，PBS和AS eye-dropp.ConclusionPRP滴眼液存储在4°C为4周促进角膜上皮伤口愈合与更高水平的生长因子比AS滴眼液中观察到的，同时保持无菌性，这表明该制剂满足未满足的医疗需求，在治疗难治性角膜结膜上皮疾病。
Background/aimsThis study aimed to assess the efficacy and sterility of stored platelet-rich plasma (PRP) eye-drops for corneal epithelial wound healing compared with those of autologous serum (AS) eye-drops.MethodsAt our single institution, PRP and AS eye-drops were prepared using peripheral blood obtained from six healthy volunteers and stored at 4°C. Platelet and leucocyte counts and transforming growth factor (TGF)-β1, epidermal growth factor (EGF), and fibronectin levels were assessed during storage for up to 4 weeks. Sterility was assessed by culturing 4-week poststorage samples. PRP, AS, and phosphate-buffered saline (PBS) eye-drop efficacies were compared using corneal epithelial wound healing assaysin vitroandin vivoand monitoring wound areas under a microscope every 3 hours.ResultsHigher platelet and lower leucocyte counts were seen in PRP than in whole blood on the day of preparation. After storage, TGF-β1, EGF, and fibronectin levels were significantly higher in PRP than in AS eye-drops.In vitroandin vivo, PRP eye-drops used on the day of preparation significantly promoted corneal epithelial wound healing compared with PBS. Moreover, PRP eye-drops stored for 4 weeks significantly promoted corneal wound healing compared with PBS and AS eye-drops.ConclusionPRP eye-drops stored at 4°C for 4 weeks promoted corneal epithelial wound healing with higher levels of growth factors than those observed in AS eye-drops, while maintaining sterility, suggesting that this preparation satisfies the unmet medical needs in the treatment of refractory keratoconjunctival epithelial disorders.