Characterization and Genome Sequencing of Phage Abp1, a New phiKMV-Like Virus Infecting Multidrug-Resistant Acinetobacter baumannii
Characterization and Genome Sequencing of Phage Abp1, a New phiKMV-Like Virus Infecting Multidrug-Resistant Acinetobacter baumannii
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DOI：
10.1007/s00284-013-0308-7
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发表时间：
2013-06-01
影响因子：
2.6
通讯作者：
Hu, Fuquan
中科院分区：
文献类型：
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作者：
Huang, Guangtao;Le, Shuai;Hu, Fuquan
在筛选多重耐药鲍曼不动杆菌的替代抗生素时，我们分离到了一株毒力较强的鲍曼不动杆菌噬菌体Abp1。透射电子显微镜显示，该噬菌体有一个二十面体的头部和一条短尾，应被归类为Podoviridae家族的成员。SDS-PAGE分析表明，Abp1含有至少1个主要蛋白和9个次要蛋白。在一步生长试验中，我们证明了Abp1的潜伏期为10min，突发量为350。Abp1的寄主范围也相对较窄。对整个基因组进行了测序，最终组装产生了一个42,185个碱基的线性双链DNA分子，G+C含量为39.15%，包含54个可能的基因。在这些基因中，有26个是功能已知的，剩下28个未知的推定基因。Abp1是T7群phiKMV样病毒亚群的新成员，其基因组序列与鲍曼不动杆菌噬菌体phiAB1非常相似。
While screening for alternative antibiotics against multidrug-resistant Acinetobacter baumannii, we isolated a virulent A. baumannii bacteriophage Abp1. Transmission electron microscopy revealed that the phage had an icosahedral head with a short tail and should be classified as a member of the Podoviridae family. SDS-PAGE showed that Abp1 contained at least one major and nine minor proteins. In a single-step growth test, we demonstrated that Abp1 had a latent period of 10 min and a burst size of 350. Abp1 also had a relatively narrow host range. The entire genome was sequenced, and the final assembly yielded a 42,185 bp, linear, double-stranded DNA molecule with a G+C content of 39.15 % and containing 54 putative genes. Among these genes, 26 were functionally known, leaving 28 unknown putative genes. Abp1 is a new member of the phiKMV-like virus subgroup of the T7 group; its genome sequence is very similar to that of the A. baumannii phage phiAB1.