Functional neuroimaging of aversive taste-related areas in the alert rat revealed by positron emission tomography
Functional neuroimaging of aversive taste-related areas in the alert rat revealed by positron emission tomography
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DOI：
10.1002/jnr.23252
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发表时间：
2013-10-01
影响因子：
4.2
通讯作者：
Koshikawa, Noriaki
中科院分区：
文献类型：
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作者：
Kobayashi, Masayuki;Cui, Yilong;Koshikawa, Noriaki
在非侵入性脑功能成像技术中，F-18-氟代脱氧葡萄糖(FDG)正电子发射断层扫描(PET)在检测自由活动动物大脑活动区域方面具有比较优势。我们开发了一种[F-18]FDG-PET方案，它可以在自由活动的大鼠中显示优先对柠檬酸盐诱导的多种行为做出反应的活跃脑区。此外，还进行了c-Fos免疫组织化学染色，以检查PET检测到的区域是否对应于c-Fos免疫阳性神经元的区域。口服柠檬酸盐(0.1M)，检测味觉反应激活的脑区和排斥行为，包括张口和舌头伸出，这将有效地激活边缘系统。从自由活动或使用蒸馏水期间获得的图像中减去柠檬酸刺激期间的PET图像。柠檬酸盐增加了多个味觉相关区域的FDG信号：伏隔核(核心和外壳)、丘脑腹内侧核、杏仁核的基底外侧核和中央核、下丘脑和岛叶皮质。此外，在味觉研究领域受到较少关注的腹外侧纹状体、扣带回和内嗅觉皮质也显示出FDG信号的增加。正如预期的那样，c-Fos免疫阳性细胞也在这些区域被发现，这表明口腔内注射柠檬酸盐诱导的FDG信号增加可能反映了这些区域的神经活动。我们的[F-18]FDG-PET方案揭示了自由活动大鼠对厌恶味觉做出反应的多个大脑区域的贡献，该方法可能有助于识别未知的神经网络，特别是与边缘信息处理相关的神经网络。(C)2013年威利期刊公司。
Among noninvasive functional brain imaging techniques, F-18-fluorodeoxyglucose (FDG)-positron emission tomography (PET) has a comparative advantage in detecting active brain regions in freely locomoting animals. We developed an [F-18]FDG-PET protocol that visualizes active brain regions that respond preferentially to citrate-induced multiple behaviors in freely locomoting rats. In addition, c-Fos immunohistochemistry, an activity-dependent mapping, was performed to examine whether the areas detected by PET correspond to regions with c-Fos-immunopositive neurons. Citrate (0.1 M) was intraorally applied to detect activated brain regions responding to gustation and the rejection behaviors including gaping and tongue protrusion, which would potently activate the limbic system. PET images during citrate stimulation were subtracted from those obtained during free locomotion or during application of distilled water. Citrate increased FDG signals in multiple gustation-related regions: the nucleus accumbens (core and shell), the ventromedial nucleus of the thalamus, the basolateral and central nuclei of the amygdala, the hypothalamus, and the insular cortex. In addition, the ventrolateral striatum and the cingulate and entorhinal cortices, which have received less attention in the field of gustatory studies, also showed an increase in FDG signals. As expected, c-Fos-immunopositive cells were also found in these regions, suggesting that increased FDG signals induced by intraoral citrate injection are likely to reflect neural activity in these regions. Our [F-18]FDG-PET protocol reveals the contributions of multiple brain regions responding to aversive taste in freely locomoting rats, and this approach may aid in the identification of unknown neural networks especially relating to the limbic information processing. (c) 2013 Wiley Periodicals, Inc.