Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections
Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections
复制标题
DOI：
10.1002/bmc.3494
复制
发表时间：
2015-12-01
影响因子：
1.8
通讯作者：
Zeiri, Yehuda
中科院分区：
文献类型：
--
作者：
Abd El Qader, Amir;Lieberman, David;Zeiri, Yehuda
呼吸道感染（RI）可以是病毒或细菌的起源。在任何一种情况下，病原体的入侵都会导致各种挥发性有机化合物（VOC）的产生和释放。本研究检测了5种病毒（甲型流感病毒、B型流感病毒、腺病毒、呼吸道合胞病毒和副流感病毒1型）、3种细菌（卡他莫拉菌、流感嗜血杆菌和嗜肺军团菌）和肺炎支原体分离菌落培养物中释放的VOC。我们的研究结果表明炎症诱导的VOCs的参与。两个重要的挥发性有机化合物被确定为与感染性细菌活性，庚烷和甲基环己烷。在以前的研究中，这两种VOC与氧化应激效应有关。为了区分细菌和病毒阳性培养物，我们进行了主成分分析，包括峰身份（保留时间）和VOC浓度（即峰下面积），表明1-己醇和1-十七烯是良好的预测因子。版权所有（c）2015约翰威利父子有限公司
Respiratory infections (RI) can be viral or bacterial in origin. In either case, the invasion of the pathogen results in production and release of various volatile organic compounds (VOCs). The present study examines the VOCs released from cultures of five viruses (influenza A, influenza B, adenovirus, respiratory syncitial virus and parainfluenza 1 virus), three bacteria (Moraxella catarrhalis, Haemophilus influenzae and Legionella pneumophila) and Mycoplasma pneumoniae isolated colonies. Our results demonstrate the involvement of inflammation-induced VOCs. Two significant VOCs were identified as associated with infectious bacterial activity, heptane and methylcyclohexane. These two VOCs have been linked in previous studies to oxidative stress effects. In order to distinguish between bacterial and viral positive cultures, we performed principal component analysis including peak identity (retention time) and VOC concentration (i.e. area under the peak) revealing 1-hexanol and 1-heptadecene to be good predictors. Copyright (c) 2015 John Wiley & Sons, Ltd.