Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization
Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization
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DOI：
10.1139/gen-41-4-580
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发表时间：
1998-08-01
期刊：
影响因子：
3.1
通讯作者：
Thomas, JB
中科院分区：
文献类型：
--
作者：
Chen, Q;Conner, RL;Thomas, JB
利用长穗偃麦草（Thinopyrum elongatum（Host）D.R.）基因组DNA探针进行基因组原位杂交（GISH）Dewey（基因组E，2n = 14）、白萨偃麦草Thinopyrum bessarabicum（Savul. & Rayss）A. Love（基因组J，2n = 14）和Pseudoroegneria strigosa（M. Bieb.）A. Love（染色体组S，2n = 14），分析了中间偃麦草（Thinopyrum intermedium（Host）Barkworth Sr D.R.）Dewey（2n = 6x = 42）和Thinopyrum ponticllm（Podp.）Barkworth Sr D.R. Dewey（2n = 10x = 70）。GISH结果表明，六倍体中间山雀含有J、J（s）和S基因组，其中J基因组与Th. elongatum和Th.比萨拉比姆。S基因组与Ps的S基因组同源。而J（s）基因组是指修饰的J-或E-型染色体，其特征在于靠近着丝粒的S基因组特异性序列的存在。十倍体ponticum只有J和J（s）两个基本基因组。Th中存在J（s）基因组。intermedium和Th. ponticum与E或J基因组同源，但在着丝粒区域有很大差异，可与S基因组DNA探针进行强杂交。根据GISH结果，推测了该菌的基因组结构式。将中间型（Th. intermedium）重新命名为JJ（s）S。ponticllm被重新命名为JJJJ（s）J（s）。S、J和J（s）基因组之间密切关系的发现为利用S基因组DNA探针进行分子细胞遗传学分析以监测有用性状从Th的转移提供了有价值的标记。intermedium和Th. ponticum到小麦。
Genomic in situ hybridization (GISH) using genomic DNA probes from Thinopyrum elongatum (Host) D.R. Dewey (genome E, 2n = 14), Thinopyrum bessarabicum (Savul. & Rayss) A. Love (genome J, 2n = 14), and Pseudoroegneria strigosa (M. Bieb.) A. Love (genome S, 2n = 14), was used to examine the genomic constitution of Thinopyrum intermedium (Host) Barkworth Sr D.R. Dewey (2n = 6x = 42) and Thinopyrum ponticllm (Podp.) Barkworth Sr D.R. Dewey (2n = 10x = 70). Evidence from GISH indicated that hexaploid Tit, intermedium contained the J, J(s), and S genomes, in which the J genome was related to the E genome of Th. elongatum and the J genome of Th. bessarabicum. The S genome was homologous to the S genome of Ps. strigosa, while the J(s) genome referred to modified J- or E-type chromosomes distinguished by the presence of S genome specific sequences close to the centromere. Decaploid Th. ponticum had only the two basic genomes J and J(s). The J(s) genome present in Th. intermedium and Th. ponticum was homologous with E or J genomes, but was quite distinct at centromeric regions, which can strongly hybridize with the S genome DNA probe. Based on GISH results, the genomic formula of Th. intermedium was redesignated JJ(s)S and that of Th. ponticllm was redesignated JJJJ(s)J(s). The finding of a close relationship among S, J, and J(s) genomes provides valuable markers for molecular cytogenetic analyses using S genome DNA probes to monitor the transfer of useful traits from Th. intermedium and Th. ponticum to wheat.