Moringa oleifera extract mediated the synthesis of Bio-SeNPs with antibacterial activity against Listeria monocytogenes and Corynebacterium diphtheriae
Moringa oleifera extract mediated the synthesis of Bio-SeNPs with antibacterial activity against Listeria monocytogenes and Corynebacterium diphtheriae
复制标题
DOI：
10.1016/j.lwt.2022.113751
复制
发表时间：
2022-07-09
影响因子：
6
通讯作者：
Xia, Xian
中科院分区：
文献类型：
--
作者：
Ao, Bo;Lv, Jing;Xia, Xian
单核细胞增生李斯特菌和白喉棒状杆菌是典型的细菌性病原菌，由于其耐药性不断增强，对食品安全和人类健康构成越来越大的威胁。以辣木提取物和亚硒酸钠为原料，制备了生物硒纳米颗粒（Bio-SeNPs）。合成的BioSeNPs用于治疗这两种病原体。TEM结果表明，它们呈球形，直径在50 ~ 200 nm之间。元素图谱、FTIR和XPS分析表明，生物硒纳米颗粒由Se（0）和M.油茶属。抑菌圈试验表明，7和0.7 mg/ mL的Bio-SeNPs对L.单核细胞增多症和C.白喉生长曲线实验和活/死细胞染色实验表明，0.7 mg/mL的Bio-SeNPs对这两株菌的生长有明显的抑制作用。扫描电镜观察表明，L.单核细胞增多症和C.用0.7mg/mL Bio-SeNPs处理后，白喉细胞皱缩并破裂。此外，Bio-SeNPs能有效清除L.单核细胞增生对生鲑鱼没有细胞毒性。特别地，本研究中的Bio-SeNPs由可食用Se（0）和M.油茶属。因此，它在食品和医药科学中显示出巨大的潜力。
Listeria monocytogenes and Corynebacterium diphtheriae are typical bacterial pathogens and pose an increasing threat to food safety and human health due to their growing antibiotic resistance. In this study, biological selenium nanoparticles (Bio-SeNPs) were synthesized by Moringa oleifera extract and selenite. The synthesized BioSeNPs were used to treat these two pathogens. They were spherical, and their diameters ranged from 50 to 200 nm according to the TEM result. The element-mapping, FTIR and XPS analyses showed that the Bio-SeNPs consisted of Se(0) and organics produced by M. oleifera. The inhibition zone tests showed that 7 and 0.7 mg/ mL of Bio-SeNPs exhibited noticeable inhibition of L. monocytogenes and C. diphtheriae. Furthermore, the growth curve experiments and live/dead cell stain experiments showed that 0.7 mg/mL of Bio-SeNPs could significantly inhibit the growth of these two strains. Morphological observation by SEM showed that L. monocytogenes and C. diphtheriae cells shriveled and broke after treatment with 0.7 mg/mL of Bio-SeNPs. Additionally, the Bio-SeNPs could efficiently clear L. monocytogenes on the raw salmon without cytotoxicity. In particular, the Bio-SeNPs in this study were composed of edible Se(0) and M. oleifera. Accordingly, it exhibited great potential in food and medicine science.