Salivary proteomics in bisphosphonate-related osteonecrosis of the jaw.
Salivary proteomics in bisphosphonate-related osteonecrosis of the jaw.
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DOI：
10.1111/odi.12204
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发表时间：
2015-01
期刊：
影响因子：
3.8
通讯作者：
Gopalakrishnan R
中科院分区：
文献类型：
--
作者：
Thumbigere-Math V;Michalowicz BS;de Jong EP;Griffin TJ;Basi DL;Hughes PJ;Tsai ML;Swenson KK;Rockwell L;Gopalakrishnan R
鉴定双磷酸盐相关颌骨坏死 (BRONJ) 患者中差异表达的唾液蛋白，这些蛋白可作为 BRONJ 诊断的生物标志物。将从 20 名 BRONJ 患者和 20 名对照组获得的全部唾液汇集到各组中。使用 iTRAQ 标记的二维液相色谱-串联质谱法对样品进行分析。总共鉴定出 1340 种蛋白质。其中，候选生物标志物的选择基于 p 值（<0.001）、蛋白质表达的变化（≥1.5 倍的增加或减少）以及识别的独特肽（≥2）。 BRONJ 和对照之间进行的三项比较确定了 BRONJ 患者中差异表达的 200 种蛋白质。大多数这些蛋白质预计在药物代谢、免疫学和皮肤病学中发挥作用。在所有差异表达的蛋白质中，我们选择了金属蛋白酶9和桥粒斑蛋白进行进一步验证。免疫测定证实 BRONJ 患者个体唾液 (p=0.048) 和血清样本 (p=0.05) 中金属蛋白酶-9 的表达增加。唾液中检测不到桥粒斑蛋白。然而，BRONJ 血清中的桥粒斑蛋白水平往往低于对照组 (p=0.157)。 BRONJ 的发育涉及多种病理反应。这项研究鉴定出的一种或多种蛋白质可能被证明是 BRONJ 诊断有用的生物标志物。金属蛋白酶 9 和桥粒斑蛋白在 BRONJ 中的作用需要进一步研究。
To identify differentially expressed salivary proteins in bisphosphonate-related osteonecrosis of the jaw (BRONJ) patients that could serve as biomarkers for BRONJ diagnosis. Whole saliva obtained from 20 BRONJ patients and 20 controls were pooled within groups. The samples were analyzed using iTRAQ-labeled two-dimensional liquid chromatography-tandem mass spectrometry. Overall, 1340 proteins were identified. Of these, biomarker candidates were selected based on p-value (<0.001), changes in protein expression (≥1.5-fold increase or decrease), and unique peptides identified (≥2). Three comparisons made between BRONJ and controls identified 200 proteins to be differentially expressed in BRONJ patients. A majority of these proteins were predicted to have a role in drug metabolism, immunological and dermatological diseases. Of all the differentially expressed proteins, we selected metalloproteinase-9 and desmoplakin for further validation. Immunoassays confirmed increased expression of metalloproteinase-9 in individual saliva (p=0.048) and serum samples (p=0.05) of BRONJ patients. Desmoplakin was undetectable in saliva. However, desmoplakin levels tended to be lower in BRONJ serum than controls (p=0.157). Multiple pathological reactions are involved in BRONJ development. One or more proteins identified by this study may prove to be useful biomarkers for BRONJ diagnosis. The role of metalloproteinase-9 and desmoplakin in BRONJ requires further investigation.
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影响因子：
7.6
作者：
Mawardi, H.;Giro, G.;Kawai, T.
通讯作者：
Kawai, T.
影响因子：
1.9
作者：
Lehrer, Steven;Montazem, Andre;Kogan, Rita
通讯作者：
Kogan, Rita
DOI：
10.14219/jada.archive.2011.0108
发表时间：
2011-11-01
影响因子：
3.9
作者：
Hellstein, John W.;Adler, Robert A.;Ristic, Helen
通讯作者：
Ristic, Helen
DOI：
10.1016/j.tripleo.2007.08.040
发表时间：
2008-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Bedogni, Alberto;Blandamura, Stella;Nocini, Pier Francesco
通讯作者：
Nocini, Pier Francesco
影响因子：
6.2
作者：
Hoff, Ana O.;Toth, Bela B.;Hortobagyi, Gabriel N.
通讯作者：
Hortobagyi, Gabriel N.