Characterization of Biogenic Iron Oxides Collected from the Newly Designed Liquid Culture Method using Diffusion Chambers
Characterization of Biogenic Iron Oxides Collected from the Newly Designed Liquid Culture Method using Diffusion Chambers
复制标题
使用扩散室从新设计的液体培养方法中收集的生物氧化铁的表征
DOI：
10.1111/gbi.12073
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
and Y. Takahashi
中科院分区：
文献类型：
--
作者：
S. Kikuchi;H. Makita;K. Takai;N. Yamaguchi;and Y. Takahashi
我们设计了一种使用液体培养基培养嗜酸性铁氧化细菌的新方法，以（i）研究生物氧化铁（BIOS）的形成和矿物学特征，（ii）将BIOS应用于各种科学和工程应用。使用一组扩散室培养铁氧化细菌Mariprofundus ferrooxydans PV-1 T（ATCC，BAA-1020）以制备宽的缺氧-好氧界面，在该界面上通常在自然环境中观察到BIOS形成。氧化铁沉淀与细菌生长平行产生。扫描电子显微镜分析表明，介质中氧化铁沉淀物的形态特征（体外BIOS）与从马里亚纳弧北方的西北Eifuku海山区天然深海热液环境中收集的BIOS（原位BIOS）相似。用X射线吸收精细结构（XAFS）研究了体外和原位BIOS的进一步化学形态。大量XAFS分析表明，这两种BIOS中的矿物主要是水铁矿和低聚阶段的无定形铁羟基氧化物与边缘共享八面体键。用扩散室方法产生的体外BIOS的量大于先前用其他培养方法产生的量，例如凝胶稳定梯度和分批液体培养方法。用新的培养方法生产的BIOS产量更高，这将使我们能够在未来澄清细菌生长过程中的矿化机制，并检查BIOS的物理化学性质，例如它们对各种元素和物质的吸附和共沉淀。
We designed a new culture method for neutrophilic iron‐oxidizing bacteria using liquid medium (i) to study the formation and mineralogical characteristics of biogenic iron oxides (BIOS) and (ii) to apply BIOS to various scientific and engineering applications. An iron‐oxidizing bacterium,Mariprofundus ferrooxydans PV‐1T(ATCC, BAA–1020), was cultured using a set of diffusion chambers to prepare a broad anoxic–oxic interface, upon which BIOS formation is typically observed in natural environments. Iron oxide precipitates were generated in parallel with bacterial growth. A scanning electron microscopy analysis indicated that the morphological features of the iron oxide precipitates in the medium (in vitroBIOS) were similar to those of BIOS collected from natural deep‐sea hydrothermal environments in the Northwest Eifuku Seamount field in the northern Mariana Arc (in situBIOS). Further chemical speciation of both thein vitroandin situBIOS was examined with X‐ray absorption fine structure (XAFS). A bulk XAFS analysis showed that the minerals in both BIOS were mainly ferrihydrite and oligomeric stages of amorphous iron oxyhydroxides with edge‐sharing octahedral linkages. The amount ofin vitroBIOS produced with the diffusion‐chamber method was greater than those produced previously with other culture methods, such as gel‐stabilized gradient and batch liquid culture methods. The larger yields of BIOS produced with the new culture method will allow us to clarify in the future the mineralization mechanisms during bacterial growth and to examine the physicochemical properties of BIOS, such as their adsorption to and coprecipitation with various elements and substances.