Mode of antiviral action of silver nanoparticles against HIV-1.
Mode of antiviral action of silver nanoparticles against HIV-1.
复制标题
DOI：
10.1186/1477-3155-8-1
复制
发表时间：
2010-01-20
影响因子：
10.2
通讯作者：
Rodriguez-Padilla C
中科院分区：
文献类型：
--
作者：
Lara HH;Ayala-Nuñez NV;Ixtepan-Turrent L;Rodriguez-Padilla C
银纳米颗粒已被证明在非细胞毒性浓度下对HIV-1发挥抗病毒活性，但其HIV抑制活性的机制尚未完全阐明。在这项研究中，银纳米粒子进行评估，以阐明其抗HIV-1的抗病毒作用模式，使用一组不同的体外试验。我们的数据表明，银纳米粒子发挥抗艾滋病毒活性在病毒复制的早期阶段，最有可能作为杀病毒剂或作为病毒进入的抑制剂。银纳米颗粒与gp 120结合的方式可防止CD 4依赖性病毒体结合、融合和感染，可作为有效的杀病毒剂对抗无细胞病毒（实验室毒株、临床分离株、T和M嗜性毒株和耐药毒株）和细胞相关病毒。此外，银纳米颗粒抑制HIV-1生命周期的进入后阶段。这些特性使它们成为一种广谱剂，不易诱导耐药性，可用于预防各种流行的HIV-1菌株。
Silver nanoparticles have proven to exert antiviral activity against HIV-1 at non-cytotoxic concentrations, but the mechanism underlying their HIV-inhibitory activity has not been not fully elucidated. In this study, silver nanoparticles are evaluated to elucidate their mode of antiviral action against HIV-1 using a panel of different in vitro assays. Our data suggest that silver nanoparticles exert anti-HIV activity at an early stage of viral replication, most likely as a virucidal agent or as an inhibitor of viral entry. Silver nanoparticles bind to gp120 in a manner that prevents CD4-dependent virion binding, fusion, and infectivity, acting as an effective virucidal agent against cell-free virus (laboratory strains, clinical isolates, T and M tropic strains, and resistant strains) and cell-associated virus. Besides, silver nanoparticles inhibit post-entry stages of the HIV-1 life cycle. These properties make them a broad-spectrum agent not prone to inducing resistance that could be used preventively against a wide variety of circulating HIV-1 strains.