Soil Colloids and Minerals Modulate Metabolic Activity of Pseudomonas putida Measured Using Microcalorimetry
Soil Colloids and Minerals Modulate Metabolic Activity of Pseudomonas putida Measured Using Microcalorimetry
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DOI：
10.1080/01490451.2013.861544
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发表时间：
2014-07
影响因子：
2.3
通讯作者：
Huayong Wu;Wenli Chen;Xingmin Rong;P. Cai;Ke Dai;Qiaoyun Huang
中科院分区：
文献类型：
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作者：
Huayong Wu;Wenli Chen;Xingmin Rong;P. Cai;Ke Dai;Qiaoyun Huang
微生物与土壤颗粒的大量相互作用对与一系列生物地球化学过程相关的微生物活动产生了根本影响。然而，土壤表面活性颗粒如何调控微生物新陈代谢却鲜有人关注。土壤胶体的组成在多大程度上改变了新陈代谢并没有得到很好的解决。本研究采用微量热法和碳素利用法研究了土壤胶体和矿物质对恶臭假单胞菌代谢活性的影响。结果表明，蒙脱石能显著提高恶臭假单胞菌的代谢活性，而高岭石、针铁矿和土壤胶体对恶臭假单胞菌的代谢活性有明显的抑制作用。腐殖质可能通过界面相互作用而不是营养补充剂来减弱土壤胶体对细菌代谢的抑制。高岭石和氧化铁含量较高的土壤对细菌活性的抑制作用可能较大。热力学方法在表征微生物活动方面提供了与其他技术不同的和补充的信息。细菌在土壤成分上的粘附量和亲和力以及代谢产物的解毒作用被归因于细菌活性的修饰。
Substantial interactions of microbes with soil particles present fundamental influences on microbial activities relevant to a series of biogeochemical processes. However, how soil surface-active particles modulate microbial metabolism has received scant attention. The extent to which composition of soil colloids alter the metabolism is not well addressed. This work examined the impacts of soil colloids and minerals on the metabolic activity of Pseudomonas putida using microcalorimetry and carbon utilization. The results showed that montmorillonite remarkably improved metabolic activity of P. putida, whereas kaolinite, goethite and soil colloids significantly inhibited the activity. Humus may weaken the inhibition of soil colloids on bacterial metabolism via interfacial interaction rather than nutrient supplements. Soils bearing higher amount of kaolinite and iron oxide may have greater depression on bacterial activity. The thermodynamic method provides different and complementary information to that from other techniques in characterizing microbial activities. The quantity and affinity for the adhesion of bacteria onto soil components together with the detoxification of metabolites were assigned to the modifications of bacterial activities.