Preparation of gold/hydroxyapatite hybrids using natural fish scale template and their effective albumin interactions
Preparation of gold/hydroxyapatite hybrids using natural fish scale template and their effective albumin interactions
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DOI：
10.1016/j.apt.2018.02.011
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发表时间：
2018-05-01
影响因子：
5.2
通讯作者：
Tagaya, Motohiro
中科院分区：
文献类型：
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作者：
Chai, Yadong;Nishikawa, Masami;Tagaya, Motohiro
在天然鱼鳞模板的多级羟基磷灰石（HAp）纳米结构中成功合成了直径为15-40 nm的金（Au）纳米颗粒（NPs），并通过Au3+离子化学吸附、还原和煅烧过程形成AuNPs/HAp杂化物。 AuNPs 尺寸以及表面等离子共振 (SPR) 吸收最大值在杂交过程中得以保留。此外，AuNPs/HAp杂化纳米结构在生物牛血清白蛋白（Ab）浓度区域（相当于细胞培养基中的1.5μM和人血液中的15.1μM）表现出优先的蛋白质吸附行为，并且AuNPs/HAp杂化物的Ab吸附平衡常数高于单独的HAp。与 AuNPs/HAp 鱼鳞相比，Ab 吸附的 AuNPs/HAp 鱼鳞的 SPR 吸收最大值发生红移。因此，我们利用鱼鳞模板合成了具有优先蛋白质吸附作用的AuNPs，这对于研究AuNPs/HAp杂化功能具有重要意义。 (C) 2018 日本粉末技术协会。由 Elsevier B.V. 和日本粉末技术协会出版。版权所有。
The gold (Au) nanoparticles (NPs) with the diameter of 15-40 nm were successfully synthesized in the hierarchical hydroxyapatite (HAp) nanostructures of natural fish scale templates, which were carried out by the Au3+ ion chemisorption, reduction and calcination processes to form the AuNPs/HAp hybrids. The AuNPs size as well as the surface plasmon resonance (SPR) absorption maximum was preserved with the hybridization process. Moreover, the AuNPs/HAp hybrid nanostructures exhibited preferential protein adsorption behavior at the biological bovine serum albumin (Ab) concentration regions that correspond to be 1.5 mu M in the cell culture medium and 15.1 mu M in human blood, and the Ab adsorption equilibrium constant of AuNPs/HAp hybrid was higher than that of the HAp alone. The SPR absorption maxima of the Ab-adsorbed AuNPs/HAp fish scales were red-shifted as compared with those of the AuNPs/HAp fish scales. Therefore, we synthesized the AuNPs using the fish scale template to exhibit the preferential protein adsorption, which will be a great significance to research the AuNPs/HAp hybrid functions. (C) 2018 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.