A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity
A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity
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用于评估 AgNP 颗粒数对细胞毒性影响的微流控表面增强拉曼光谱方法
DOI：
10.1016/j.ecoenv.2018.07.026
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发表时间：
2018
影响因子：
6.8
通讯作者：
Qing Huang
中科院分区：
文献类型：
--
作者：
Zhimin Zhai;Mengyue Nie;Yong Guan;Fengqiu Zhang;Liang Chen;Muhammad;Wenbin Du;Gang Liu;Yangchao Tian;Qing Huang
银纳米粒子(Ag NPs)具有广为人知的抗菌性能，广泛应用于各种医疗产品和普通商品中。虽然许多研究都提到了纳米银对哺乳动物细胞的毒性，但活细胞中纳米银的数量与相应的细胞毒性之间的直接关系还没有明确的证明。在本工作中，一种简单且可重复使用的微流控装置被用来分离和捕获单个活细胞。该装置由石英盖片和带有刻蚀微通道和微孔的玻璃板组成。该装置被硅烷化以使表面疏水。为简单起见，研究中使用HeLa细胞作为癌细胞模型，将其吸管加载到基于死端填充的微孔阵列中。然后用表面增强拉曼光谱(SERS)检测活的癌细胞，并评估细胞内银纳米粒的数量和分布。因此，结合细胞活力测定，我们将细胞内的银纳米颗粒的数量与对细胞的毒性直接相关。
Silver nanoparticles (Ag NPs) have well-known antibacterial properties and are widely applied in various medical products and general commodities. Although many studies have addressed the toxicity of Ag NPs to mammalian cells, the direct relationship between the number of Ag NPs in living cells and the corresponding cell toxicity has not yet been explicitly demonstrated. In this work, a simple and reusable microfluidic device composed of a quartz cover slip and a glass plate with etched micro-channel and micro-wells was employed for separating and trapping single living cells. The device was silanized to render the surface hydrophobic. For simplicity, HeLa cells as the model cancer cells were used in the study, which were pipette-loaded into an array of micro wells based on dead-end filling. Surface enhanced Raman spectroscopy (SERS) was then employed to examine the living cancer cells and assessed number and distribution of Ag NPs in the cells. Combined with the cell viability assay, we therefore correlated the number of Ag NPs in the cell with the toxicity to the cell directly.