RD 114 Virus-Specific Sequences in Feline Cellular RNA: Detection and Characterization
RD 114 Virus-Specific Sequences in Feline Cellular RNA: Detection and Characterization
复制标题
猫细胞 RNA 中的 RD 114 病毒特异性序列：检测和表征
作者：
H. Okabe;R. Gilden;M. Hatanaka
从猫细胞中提取的RNA含有与RD-114病毒RNA同源的序列。以内源性病毒RNA为模板，用病毒粒子聚合酶合成的单链DNA探针进行分子杂交。迄今为止，已在所有猫源性细胞中检测到病毒特异性RNA，但在其他动物细胞中未检测到，除了产生病毒的人横纹肌肉瘤细胞RD-114。DNA探针与细胞RNA的杂交程度与与病毒70S RNA的杂交程度相当，这表明在所有猫细胞以及RD-114细胞中都存在相同程度的病毒特异性序列。在猫细胞中，这种病毒RNA的数量达到每个细胞约100个拷贝，而产生病毒的RD-114细胞每个细胞约含有1000个拷贝。病毒RNA在猫细胞中以35S和18S两种不同的大小存在，而在RD-114细胞中，病毒特异性RNA在大小上是相当不同的。
RNA extracted from cat cells contains sequences homologous to RD-114 viral RNA. The sequences are measured by molecular hybridization with a single-stranded DNA probe synthesized by the virion polymerase using the endogenous viral RNA as template. Viral-specific RNA has been detected in all cells of cat origin tested thus far, but not in cells of other animals, except for the virus-producing human rhabdomyosarcoma cell, RD-114. The extent of hybridization of the DNA probe to cellular RNA was equivalent to that obtained with viral 70S RNA indicating that an equal extent of viral specific sequences is present in all cat cells as well as in RD-114 cells. The amounts of this viral RNA reach approximately 100 copies per cell in cat cells, while virus-producing RD-114 cells contain about 1,000 copies per cell. The viral RNA is present in cat cells in two distinct sizes of about 35S and 18S, whereas in RD-114 cells virus-specific RNA is quite heterogeneous in size.