Differential expression analysis of genes involved in high-temperature induced sex differentiation in Nile tilapia
Differential expression analysis of genes involved in high-temperature induced sex differentiation in Nile tilapia
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DOI：
10.1016/j.cbpb.2014.08.006
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发表时间：
2014-11-01
影响因子：
2.2
通讯作者：
Ji, Xiang Shan
中科院分区：
文献类型：
--
作者：
Li, Chun Ge;Wang, Hui;Ji, Xiang Shan
目前，高温对硬骨鱼类性别分化的分子途径的影响还有待进一步研究。本研究对高温诱导尼罗罗非鱼性腺和脑性别分化相关基因进行了系统的差异表达分析。结果表明，高温诱导组雌雄性腺中CYP19A1A表达显著下调，诱导组卵巢中FOXL2表达显著下调。在诱导组和恢复组，GTH α、LH β和ER α的表达在两性脑中也显著下调。相反，CYP11B2在卵巢中的表达显著上调，而在睾丸中的表达在两组中均不显著。斯皮尔曼等级相关分析表明，CYP19 A1A、FOXL2、DMRT 1在性腺中的表达与脑中某些基因的表达存在显著相关性。本研究的另一个结果显示，高温上调了诱导组睾丸中DNMT 1的表达水平，以及两组雌性脑中DNMT 1和DNMT 3A的表达水平。热休克蛋白的表达及相关性分析表明，高温对罗非鱼热休克蛋白的作用可能间接引起性腺性别分化相关基因的表达变化。这些发现为鱼类TSD的研究提供了新的思路，并表明性别分化相关基因、热休克蛋白和DNA甲基化基因是研究鱼类TSD的新候选基因。(C)2014爱思唯尔公司All rights reserved.
Nowadays, high temperature effects on the molecular pathways during sex differentiation in teleosts need to be deciphered. In this study, a systematic differential expression analysis of genes involved in high temperature-induced sex differentiation was done in the Nile tilapia gonad and brain. Our results showed that high temperature caused significant down-regulation of CYP19A1A in the gonad of both sexes in induction group, and FOXL2 in the ovary of the induction group. The expressions of GTH alpha, LH beta and ER alpha were also significantly down-regulated in the brain of both sexes in the induction and recovery groups. On the contrary, the expression of CYP11B2 was significantly up-regulated in the ovary, but not in the testis in both groups. Spearman rank correlation analysis showed that there are significant correlations between the expressions of CYP19A1A, FOXL2, or DMRT1 in the gonads and the expression of some genes in the brain. Another result in this study showed that high temperature up-regulated the expression level of DNMT1 in the testis of the induction group, and DNMT1 and DNMT3A in the female brain of both groups. The expression and correlation analysis of HSPs showed that high temperature action on tilapia HSPs might indirectly induce the expression changes of sex differentiation genes in the gonads. These findings provide new insights on TSD and suggest that sex differentiation-related genes, heat shock proteins, and DNA methylation genes are new candidates for studying TSD in fish species. (C) 2014 Elsevier Inc. All rights reserved.