Photo-induced DNA cleavage by zinc-substituted myoglobin with a redesigned active center
Photo-induced DNA cleavage by zinc-substituted myoglobin with a redesigned active center
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重新设计活性中心的锌取代肌红蛋白诱导光诱导 DNA 裂解
DOI：
10.1039/c7qi00384f
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发表时间：
2017-12-01
影响因子：
7
通讯作者：
Lin, Ying-Wu
中科院分区：
文献类型：
--
作者：
Shi, Zhen-Hua;Du, Ke-Jie;Lin, Ying-Wu
肌红蛋白与重新设计的活性中心的构建和重建与锌原卟啉（ZnPP），这是表现出新的光诱导的DNA切割活动，涉及羟基自由基（stecOH）与单线态氧（1O2）的贡献较小，证实了自旋捕获研究的第一次。
Myoglobin with a redesigned active center was constructed and reconstituted with zinc protoporphyrin (ZnPP), which was shown to exhibit novel photo-induced DNA cleavage activities, involving a hydroxyl radical (˙OH) with a minor contribution from singlet oxygen (1O2), as confirmed by spin-trapping studies for the first time.