Microrna-1224-5p Is a Potential Prognostic and Therapeutic Biomarker in Glioblastoma: Integrating Bioinformatics and Clinical Analyses
Microrna-1224-5p Is a Potential Prognostic and Therapeutic Biomarker in Glioblastoma: Integrating Bioinformatics and Clinical Analyses
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Microrna-1224-5p 是胶质母细胞瘤的潜在预后和治疗生物标志物：整合生物信息学和临床分析
DOI：
10.1007/s11596-022-2593-5
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发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Bin Luo
中科院分区：
文献类型：
--
作者：
Xing Wei;Qing-mei Zhang;Chang Liu;Song Wu;Wei-xia Nong;Ying-ying Ge;Li-na Lin;Feng Li;Xiao-xun Xie;Bin Luo
胶质母细胞瘤（GBM）是最常见的侵袭性恶性原发性脑肿瘤，预后差，复发率高。已知一些与肿瘤发生、发展相关的microRNA（miRNAs）可作为肿瘤（包括胶质母细胞瘤）预后和治疗的靶点。本研究的目的是突出GBM的核心miRNA的潜在作用，并作为一个预后和治疗的biological.MethodsDifferentially表达的miRNA（DEmiRNA）的潜在用途被确定在GBM通过整合miRNA测序结果和GBM微阵列数据集从基因表达Omnibus（GEO）数据库，通过生物信息学工具。通过中国胶质瘤基因组图谱（CGGA）进行生存分析，鉴定表达异常的miRNAs。在MiRWalk和miRTarBase数据库中预测表达失调的miRNAs的靶基因。利用TAM 2. 0数据库、基因本体（GO）和京都基因与基因组百科全书（KEGG）途径分析功能异常的miRNAs。随后，使用蛋白质-蛋白质相互作用（PPI）网络分析来分别鉴定上调和下调的miRNA的前20个中心靶标。然后，通过构建调控异常的miRNA-differentially expressed hub基因网络，鉴定GBM中的核心miRNAs。与8个正常脑组织相比，在41个GBM组织中检测到核心miRNA表达的验证。结果共检测到68个DEmiRNAs，其中40个表达上调，28个表达下调。2个上调和4个下调的miRNAs具有预后意义。大多数差异表达的hub基因受miR-28- 5 p和miR-1224- 5 p的调控，它们在GBM中分别上调和下调。miR-1224- 5 p水平与复发的相关性具有统计学意义（P=0.011）。生存分析显示高水平miR-28- 5 p和高水平miR-1224- 5 p均与较好的预后相关。结论miR-1224 - 5 p可能成为GBM患者预后和治疗的潜在靶点。
ObjectiveGlioblastoma (GBM) is the most common, invasive, and malignant primary brain tumor with a poor prognosis and high recurrence rate. It’s known that some microRNAs (miRNAs) which are associated with tumorigenesis and progression can be considered as prognostic and therapeutic targets in tumors including GBM. This study aims to highlight the potential role of the core miRNAs in GBM and their potential use as a prognostic and therapeutic biomarker.MethodsDifferentially expressed miRNAs (DEmiRNAs) were identified in GBM by integrating miRNA-sequencing results and a GBM microarray dataset from the Gene Expression Omnibus (GEO) database through bioinformatics tools. The dysregulated miRNAs were identified by survival analysis through Chinese Glioma Genome Atlas (CGGA). Target genes of the dysregulated miRNAs were predicted on MiRWalk and miRTarBase database. TAM2.0 database, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis were used to analyze the function of the dysregulated miRNAs. Subsequently, protein-protein interaction (PPI) network analysis was used to identify the top 20 hub targets of the up-regulated and down-regulated miRNAs, respectively. Then, core miRNAs in GBM were identified by constructing dysregulated miRNA-differentially expressed hub gene networks. Validation of the core miRNAs expression was detected in 41 GBM tissues compared to 8 normal brain tissues. Furthermore, the potential biomarkers were identified by clinical correlation analysis and survival analysis.ResultsTotally, 68 intersecting DEmiRNAs were identified, 40 of which were upregulated and the other 28 miRNAs were downregulated. Two upregulated and 4 downregulated miRNAs showed prognostic significance. Most differentially expressed hub genes were regulated by the miR-28-5p and miR-1224-5p, which were respectively upregulated and downregulated in GBM. The correlation between miR-1224-5p level and recurrence was statistically significant (P=0.011). Survival analysis showed that high miR-28-5p level and high miR-1224-5p level were both associated with better prognosis. Moreover, high miR-1224-5p level was an independent prognosis factor for GBM patients according to the cox regression analysis.ConclusionMiRNA-1224-5p could be a potential target for the prognosis and treatment in GBM.
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影响因子：
--
作者：
Szopa W;Burley TA;Kramer-Marek G;Kaspera W
通讯作者：
Kaspera W
MicroRNA 与癌症：分子治疗的关键范例
DOI：
--
发表时间：
2018
期刊：
Oncol Lett
影响因子：
--
作者：
Tan W;Liu B;Qu S;Liang G;Luo W;Gong C
通讯作者：
Gong C
microRNA 在人类癌症中的作用
影响因子：
39.3
作者：
Yong Peng;Carlo M. Croce
通讯作者：
Carlo M. Croce
DOI：
10.3390/ph13110389
发表时间：
2020-11-14
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Khaddour K;Johanns TM;Ansstas G
通讯作者：
Ansstas G
影响因子：
4
作者：
Shea A;Harish V;Afzal Z;Chijioke J;Kedir H;Dusmatova S;Roy A;Ramalinga M;Harris B;Blancato J;Verma M;Kumar D
通讯作者：
Kumar D