Probing the interior of synaptic vesicles with internalized nanoparticles.
Probing the interior of synaptic vesicles with internalized nanoparticles.
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用内化纳米颗粒探测突触小泡的内部。
DOI：
10.1117/12.905091
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Chiu,DanielT
中科院分区：
文献类型：
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作者：
Gadd,JenniferC;Budzinski,KristiL;Chan,Yang-Hsiang;Ye,Fangmao;Chiu,DanielT
突触囊泡是存在于突触终扣中的亚细胞器，负责神经元之间的信号传播。突触囊泡与神经元膜进行胞内和胞吐作用以装载和释放神经递质。在这里，我们将讨论如何利用这一特性来加载纳米粒子作为探测突触囊泡内部的一种手段。为了探测突触囊泡的囊泡内区域，我们开发了一种高度敏感的pH传感聚合物点。我们认为pH敏感聚合物点的强大性质将提供对质子加载到突触囊泡中的动力学的洞察。
Synaptic vesicles are subcellular organelles that are found in the synaptic bouton and are responsible for the propagation of signals between neurons. Synaptic vesicles undergo endo- and exocytosis with the neuronal membrane to load and release neurotransmitters. Here we discuss how we utilize this property to load nanoparticles as a means of probing the interior of synaptic vesicles. To probe the intravesicular region of synaptic vesicles, we have developed a highly sensitive pH-sensing polymer dot. We feel the robust nature of the pH-sensing polymer dot will provide insight into the dynamics of proton loading into synaptic vesicles.